Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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GR37
Sigma-AldrichAnti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).
More>>This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes
Frozen Sections (1:50-1:100) Gel Shift (see comments) Immunoblotting (1:200-1:400) Immunoprecipitation (10 µl/mg protein lysate) Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Application Comments
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified, chick intestine vitamin D receptor
Immunogen
Chicken
Clone
9A7γE10.4
Host
Rat
Isotype
IgG₁
Species Reactivity
Amphibian
Chicken
Hamster
Human
Mouse
Porcine
Rat
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number
GTIN
GR37-100UG
04055977228311
Documentation
Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) SDS
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-August-2007 RFH
Application
Frozen Sections (1:50-1:100) Gel Shift (see comments) Immunoblotting (1:200-1:400) Immunoprecipitation (10 µl/mg protein lysate) Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Description
Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
Background
Vitamin D Receptor (VDR) also known as 1,25-dihydroxyvitamin D3 receptor is a member of the nuclear steroid hormone receptor superfamily which regulate gene transcription by interacting with response elements in gene promoters. VDR binds with the retinoid X receptor (RXR) from specific DNA sequences in the promoter region of vitamin D response elements (VDREs). VDR has been implicated in a variety of physiological functions including effects on growth and differentiation in normal and malignant cells. These effects are mediated through a single ligand, calcitriol. Cacitriol the active metabolite of vitamin D is a known regulator of calcium homeostasis but it also has numerous other functions including inhibition of proliferation of cancer cells and suppression of T-cell proliferation and cytokine production. Human breast carcinomas, leukemias, and prostatic carcinoma cells all express vitamin D receptor, suggesting that vitamin D may have beneficial actions on various malignancies that may prove useful in chemoprevention and/or differentiation therapy.
Host
Rat
Immunogen species
Chicken
Immunogen
purified, chick intestine vitamin D receptor
Clone
9A7γE10.4
Isotype
IgG₁
Species
amphibian, chicken, hamster, human, mouse, porcine, rat
Positive control
Breast carcinoma tissue (see comments)
Form
Liquid
Formulation
In 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11. Fleet, J.C. 1999. Nutr. Rev.57 60. Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591. Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335. Issa, L.L., et al. 1998. Inflamm. Res.47, 451. Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121. Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
