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05-386 Anti-Vinculin Antibody, clone V284

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05-386
200 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, R, RbIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-386
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Vinculin Antibody, clone V284
      Product Information
      FormatPurified
      Control
      • includes non-stimulated A431 cell lysate as a positive control, cat. no. 12-301
      PresentationPBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
      Quality LevelMQ100
      Applications
      ApplicationAnti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenHuman Vinculin
      Cloneclone V284
      HostMouse
      SpecificityVinculin
      IsotypeIgG1
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein.
      Gene Symbol
      • VCL
      • Metavinculin
      • OTTHUMP00000043885
      • vinculin
      • MVCL
      • Vinculin
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18206 # Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.
      SIZE: 1134 amino acids; 123799 Da
      SUBUNIT: Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Note=Cytoplasmic face of adhesion plaques.
      TISSUE SPECIFICITY: Metavinculin is muscle-specific.
      PTM: Phosphorylated on serines, threonines and tyrosines and acylated by myristic acid and/or palmitic acid.
      DISEASE:SwissProt: P18206 # Defects in VCL are a cause of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM).
      SIMILARITY: SwissProt: P18206 ## Belongs to the vinculin/alpha-catenin family.
      Molecular Weight117kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-386 04053252268854