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ABS82-AF647 Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate

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Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: VEGFA, VEGF UniProt Number: P15692
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ABS82-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue NumberABS82-AF647
      DescriptionAnti-VEGF Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Vascular endothelial growth factor A
      • VEGF-A
      • Vascular permeability factor
      • VPF
      • VEGF, Alexa Fluor® 647 Conjugate
      Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes VEGF at the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Non-Human Primate based on 100% sequence homology. Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology). Predicted to react with primate based on 100% sequence homology..
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VEGFA
      • VEGF
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~55 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      ABS82-AF647 04055977311945