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MAB3839-I-25UG Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3

Anti-Transglutaminase-2, FN binding domain, clone 4G3, Cat. No. MAB3839-I, is a mouse monoclonal antibody that detects Protein-glutamine gamma-glutamyltransferase 2 and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: TGM2 UniProt Number: P21980
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MAB3839-I-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3839-I-25UG
      DescriptionAnti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3
      Alternate Names
      • Protein-glutamine gamma-glutamyltransferase 2
      • EC: 2.3.2.13
      • Tissue transglutaminase
      • Transglutaminase C
      • TG(C)
      • TGC
      • TGase C
      • Transglutaminase H
      • TGase H
      • TGase-2
      Background InformationProtein-glutamine gamma-glutamyltransferase 2 (UniProt: P21980; also known as EC: 2.3.2.13, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TG2) is encoded by D13the TGM2 gene (Gene ID: 7052) in human. TG2 is a multi-domain, multi-functional enzyme that is shown to post-translationally modify proteins by catalyzing the formation of intermolecular isopeptide bonds between glutamine and lysine side-chains. It catalyes the calcium-dependent covalent modification of protein-bound glutamine side chains through transamidation or deamidation reactions. Recycling endosomes is reported to be essential for externalization of TG2, which does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. Inactivation of recycling endosomes or blocking endosome fusion with the plasma membrane is shown to block TG2 secretion. TG2 plays a critical role in extracellular matrix formation and integrin mediated signaling. It is also reported to be involved in cell signaling involving 7-transmembrane receptors. It also participates in the pathogenesis of neurodegenerative diseases and certain cancers. (Ref.: Zemskov, EA., et al (2011). PLoS ONE 6(4): e19414; Siegal, M., and Khosla, C. (2007). Pharmacol. Ther. 115(2); 232-245.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-Transglutaminase-2, FN binding domain, clone 4G3, Cat. No. MAB3839-I, is a mouse monoclonal antibody that detects Protein-glutamine gamma-glutamyltransferase 2 and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: 4 µg/mL from a representative lot detected Transglutaminase-2, FN binding domain in HUVEC lysate.

      Western Blotting Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Western Blotting applications (Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19).

      Radioimmunoassay Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Radioimmunoassay applications (Akimov, S.S., et. al. (2001). Blood. 98(5):1567-76).

      Flow Cytometry Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Flow Cytometry applications (Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Transglutaminase-2, FN binding domain in Immunoprecipitation applications (Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414; Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19).

      Immunofluorescence Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Immunofluorescence applications (Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19; Akimov, S.S., et. al. (2001). Blood. 98(5):1567-76; Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414).
      Biological Information
      ImmunogenA full-length purified tissue transglutaminase (TG-2) from human erythrocytes.
      Clone4G3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4G3 specifically detects human Transglutaminase-2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TGM2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 kDa observed; 77.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Transglutaminase-2, FN binding domain in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3839-I-25UG 04054839531927