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MABN2644-100UG Anti-Tau (MTBR3R) Antibody, clone 4B5

Anti-Tau (MTBR3R), clone 4B5, Cat. No. MABN2644, is a mouse monoclonal antibody that detects Tau protein and is tested for use in ELISA, Western Blotting, and Immunohistochemistry.

NewMABN2644-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2644-100UG
      DescriptionAnti-Tau (MTBR3R) Antibody, clone 4B5
      Alternate Names
      • Neurofibrillary tangle protein
      • Paired helical filament-tau (PHF-tau)
      • MAPTL
      • MTBT1
      • TAU
      Background InformationMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein Paired helical filament-tau (PHF-tau)) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau is a highly abundant protein found predominantly in the nervous system. Its primary role is to stabilize and support microtubule assembly, which are essential for maintaining the structure and function of nerve cells. It is present throughout the brain, particularly in regions involved in memory and cognition. MAPT gene consists of multiple exons that undergo alternative splicing, resulting in the generation of different isoforms that differ in the number and arrangement of repeats in the microtubule-binding domain. The expression of the MAPT transcripts varies and depends on the stage of neuronal maturation and neuron type. Aberrant aggregation and accumulation of tau protein have been linked to several neurodegenerative diseases collectively known as tauopathies. The most common tauopathy is Alzheimer's disease, where hyperphosphorylated tau protein forms neurofibrillary tangles inside neurons. Other tauopathies include frontotemporal dementia, progressive supranuclear palsy, and cortico-basal degeneration. These diseases are characterized by cognitive decline, motor disturbances, and memory loss.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Tau (MTBR3R), clone 4B5, Cat. No. MABN2644, is a mouse monoclonal antibody that detects Tau protein and is tested for use in ELISA, Western Blotting, and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected Tau (MTBR3R) in Western Blotting application (Kanaan, N.M., et al. (2020). Nat Commun. 11(1):2809).

      ELISA Analysis: A representative lot detected Tau (MTBR3R) in ELISA application (Kanaan, N.M., et al. (2020). Nat Commun. 11(1):2809).

      ELISA Analysis: A representative lot detected Tau recombinant protein, isoform 2N4R (blue line) and 2N3R (red line) (Data courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).

      Immunohistochemistry Applications: A representative lot detected Tau (MTBR3R) in Immunohistochemistry applications in human temporal cortex tissues from an Alzheimer s disease patient (Data courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).

      Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant, human Tau protein, isoform 2N4R.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 27 amino acids from the C-terminal half of human Tau.
      EpitopeC-terminal half
      Clone4B5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 4B5 is a mouse monoclonal antibody that detects Tau. It targets an epitope within 27 amino acids from the C-terminal half. It preferentially reacts with 3R tau isoforms, however, it also displays slight reactivity with 4R tau isoforms.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~60 kDa observed. 78.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant, human Tau, isoform 2N3R.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant, human Tau protein, isoform 2N3R.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2644-100UG 04065272074193