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05-615-I-25UL Anti-SUV39H1 Antibody, clone MG44

Anti-SUV39H1, clone MG44, Cat. No. 05-615-I, is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

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05-615-I-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue Number05-615-I-25UL
      DescriptionAnti-SUV39H1 Antibody, clone MG44
      Alternate Names
      • Histone-lysine N-methyltransferase SUV39H1
      • EC:2.1.1.355
      • Histone H3-K9 methyltransferase 1
      • H3-K9-HMTase 1
      • Lysine N-methyltransferase 1A
      • Position-effect variegation 3-9 homolog
      • Suppressor of variegation 3-9 homolog 1
      • Su(var)3-9 homolog 1
      Background InformationHistone-lysine N-methyltransferase SUV39H1 (UniProt: O43463; also known as EC:2.1.1.355, Histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, Lysine N-methyltransferase 1A, Position-effect variegation 3-9 homolog, Suppressor of variegation 3-9 homolog 1, Su(var)3-9 homolog 1, SUV39H1) is encoded by the SUV39H1 (also known as KMT1A, SUV39H) gene (Gene ID: 6839) in human. SUV39H1 specifically trimethylates lysine 9 of histone H3 using monomethylated H3 lysine 9 as the substrate. It is also reported to weakly methylate histone H1 in vitro. Trimethylation of histone H3 at lysine 9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SUV39H1 mainly functions in heterochromatin regions and plays a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. SUV39H1 is shown to be targeted to histone H3 via its interaction with RB1 and is involved in several key processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, and regulation of telomere length. It can undergo phosphorylation at serine residues, and to a lesser degree, on threonine residues and the phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. SUV39H1 contains a SET domain (aa 243-366) that contains the active site, however, both the pre-SET (aa 179-240) and post-SET 9 (aa 396-412) domains are required for the methyltransferase activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SUV39H1, clone MG44, Cat. No. 05-615-I, is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUV39H1 in U2OS and COS-7 cell lysates.

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected SUV39H1 in Chromatin Immunoprecipitation applications (Giuseppina, P., et. al. (2017). Nat. Commun. 8:15622).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated SUV39H1 in Immunoprecipitation applications (Giuseppina, P., et. al. (2017). Nat. Commun. 8:15622).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenMBP-tagged full-length recombinant human Histone-lysine N-methyltransferase SUV39H1.
      Epitopeunknown
      CloneMG44
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone MG44 is a mouse monoclonal antibody that detects Histone-lysine N-methyltransferase SUV39H1.
      IsotypeIgG
      Species Reactivity
      • Monkey
      • Human
      Species Reactivity NoteMonkey, Human. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SUV39H1
      • KMT1A
      • SUV39H
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 kDa observed; 47.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SUV39H1 in HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-615-I-25UL 04061841878308

      Documentation

      Anti-SUV39H1 Antibody, clone MG44 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SUV39H1 Antibody, clone MG44 Certificates of Analysis

      TitleLot Number
      Anti-SUV39H1, clone MG44 - Q3517675 Q3517675

      References

      Reference overviewPub Med ID
      A promoter-proximal transcript targeted by genetic polymorphism controls E-cadherin silencing in human cancers
      Giuseppina Pisignano 1 , Sara Napoli 1 , Marco Magistri 1 , Sarah N Mapelli 1 , Chiara Pastori 1 , Stefano Di Marco 1 , Gianluca Civenni 1 , Domenico Albino 1 , Claudia Enriquez 1 , Sara Allegrini 1 , Abhishek Mitra 1 , Gioacchino D'Ambrosio 2 , Maurizia Mello-Grand 3 , Giovanna Chiorino 3 , Ramon Garcia-Escudero 1 4 , Gabriele Varani 5 , Giuseppina M Carbone 1 , Carlo V Catapano
      Nat Commun  8  15622  2017

      Show Abstract       		28555645 28555645