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ABE1798 Anti-SMUG1

Anti-SMUG1, Cat. No. ABE1798, is a highly specific rabbit polyclonal antibody that targets single-strand selective monofunctional uracil DNA glycosylase and has been tested in Western Blotting.

Anti-SMUG1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMUG1 UniProt Number: Q53HV7
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ABE1798
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1798
      DescriptionAnti-SMUG1
      Alternate Names
      • Single-strand selective monofunctional uracil DNA glycosylase
      • hSMUG1
      Background InformationSingle-strand selective monofunctional uracil DNA glycosylase (UniProt: Q53HV7; also known as SMUG1, hSMUG1) is encoded by the SMUG1 gene (Gene ID: 23583) in human. SMUG1 is a base excision repair enzyme that removes uracil and oxidized pyrimidines from DNA. It recognizes base lesions in the genome and initiates base excision DNA repair. It displays greater activity toward mismatches (U/G) compared to matches (U/A). SMUG1 excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. Human and rat SMUG1 exhibit about 84% sequence homology and mouse SMUG1 has 94% homology with rat SMUG1. SMUG1 was originally identified as a monofunctional uracil-DNA glycosylase selective for ssDNA, however, later it was shown to be active for U in dsDNA. SMUG1 displays the following substrate preference: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration; and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. SMUG1 preferentially binds to a dsDNA substrate over an ssDNA substrate when both are present. SMUG1 is shown to be a pseudouridine synthase dyskerin (DKC1) partner that contributes to rRNA quality control, partly by regulating 5-hydroxymethyluridine levels. (Ref: Matsubara, M et al. (2003). Biochemistry 42(17): 4993-5002; Masaoka A., et al. (2003). Biochemistry. 42(17): 5003-5012; Jobert, L et al. (2013). Mol. Cell 49(2): 339-345).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SMUG1, Cat. No. ABE1798, is a highly specific rabbit polyclonal antibody that targets single-strand selective monofunctional uracil DNA glycosylase and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human SMUG1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects SMUG1 in HeLa cell nuclear extract. It targets an epitope within 13 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Rhesus Macaque based on high sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMUG1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~34 kDa observed; 29.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell nuclear extract.

      Western Blotting Analysis: 2 µg/mL of this antibody detected SMUG1 in 10 µg of HeLa cell nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1798 04054839193590