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MABC683 Anti-SMAD6 Antibody, clone 5H3

Detect Smad using this mouse monoclonal antibody, Anti-SMAD6 Antibody & clone 5H3 validated for use in western blotting.

Anti-SMAD6 Antibody, clone 5H3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MADH6, SMAD6 UniProt Number: O43541
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MABC683
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC683
      DescriptionAnti-SMAD6 Antibody, clone 5H3
      Alternate Names
      • Mothers against decapentaplegic homolog 6
      • MAD homolog 6
      • Mothers against DPP homolog 6
      • SMAD family member 6
      • SMAD 6
      • Smad6
      • hSMAD6
      Background InformationSMAD6 is the gene name for a protein that is a homolog of the fly gene Mothers against decapentaplegic, and in humans it is called Mothers against decapentaplegic homolog 6 (MAD homolog 6). Other names include SMAD family member 6 and Mothers against DPP homolog 6 and another gene name of MADH6. By whatever name, the protein is a transcription factor that binds to regulatory elements within the nucleus and mediates transcription. The effect is to act as a mediator of TGF-beta and BMP mediated anti-inflammatory signaling activity. SMAD6 suppresses IL1R-TLR signaling, thus preventing NF-kappa B activation and activation of proinflammatory genes. SMAD6 interacts with numerous other intracellular proteins including NEDD4L, STAMBP, PRKK, RNF111, and AXIN1. SMAD6 is expressed in a wide variety of tissues with higher levels being seen in lung and cardiac tissues. Recently SMAD6 has been shown to promote neuronal differentiation by inhibiting the Wnt/beta catenin signaling pathway in addition to inhibiting the BMP signaling. Finally in cancer SMAD6 via its suppression of TGF-beta induced growth inhibition can potentiate the transformed phenotype. Moreover SMAD6 is often over expressed in many cancers, which suppresses G0-G1 arrest and apoptosis which might otherwise normally occur, thus leaving SMAD6 playing a critical role in supporting cancer cell growth and survival. EMD-Millipore’s Anti-SMAD6 mouse monoclonal antibody has been tested in western blot against human A431, HeLa, and Jurkat cell lysates.
      References
      Product Information
      FormatAscites
      Control
      • A431, HeLa, and Jurkat cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Smad using this mouse monoclonal antibody, Anti-SMAD6 Antibody & clone 5H3 validated for use in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPurified recombinant fragment of human SMAD6 expressed in E. Coli.
      Clone5H3
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MADH6
      • SMAD6
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431, HeLa, and Jurkat cell lysates.

      Western Blot Analysis: A 1:500-2000 dilution of this antibody detected SMAD6 in A431, HeLa, and Jurkat cell lysates.

      Optimal working dilutions must be determined by end user.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC683 04053252914508