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09-843 Anti-SIRT2 Antibody

Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.

Anti-SIRT2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: SIR2, SIR2-like, SIR2L, SIR2L2 UniProt Number: Q8IXJ6
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09-843
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-843
      Replaces04-1124
      DescriptionAnti-SIRT2 Antibody
      Alternate Names
      • SIR2-like protein 2
      • silencing information regulator 2-like
      • silent information regulator 2
      • sir2-related protein type 2
      • sirtuin (silent mating type information regulation 2
      • homolog) 2 (S. cerevisiae)
        sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2
      • sirtuin 2
      • sirtuin type 2
      Background InformationSirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human brain tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate.

      Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.
      EpitopeMature protein
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the mature protein of SIRT2.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
      Gene Symbol
      • SIR2
      • SIR2-like
      • SIR2L
      • SIR2L2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.

      CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

      COFACTOR: Binds 1 zinc ion per subunit.

      ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.

      SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules.

      TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.

      DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.

      PTM: Phosphorylated at the G2/M transition of the cell cycle.

      SEQUENCE SIMILARITIES: Belongs to the sirtuin family.

      Contains 1 deacetylase sirtuin-type domain.

      SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
      Molecular WeightObserved molecular weight is ~39 kDa. Multiple isoforms around 40 kDa may be observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

      Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      09-843 04053252478468