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DR1052 Anti-SALL4 Mouse mAb (6E3)

This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Anti-SALL4 Mouse mAb (6E3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SAL-Like 4

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DR1052
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Pricing & Availability

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DR1052-100UG
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      Description
      OverviewRecognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
      Catalogue NumberDR1052
      Brand Family Calbiochem®
      SynonymsAnti-SAL-Like 4
      Application Data
      Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
      Forte, A., et al. 2009. Mol. Med. 15 392.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.2 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
      ImmunogenNA
      Clone6E3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1052-100UG 04055977225983

      Documentation

      Anti-SALL4 Mouse mAb (6E3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SALL4 Mouse mAb (6E3) Certificates of Analysis

      TitleLot Number
      DR1052

      References

      Reference overview
      Cauffman, G., et al. 2009. Hum. Reprod. 24 63.
      Forte, A., et al. 2009. Mol. Med. 15 392.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2015 JSW
      SynonymsAnti-SAL-Like 4
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (0.2 µg/ml)
      Application Data
      Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
      BackgroundThis protein may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
      Clone6E3
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
      Forte, A., et al. 2009. Mol. Med. 15 392.