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MAB1527 Anti-Peripherin Antibody, clone 8G2

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MAB1527
300 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, RICC, IHC, WBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMAB1527
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Peripherin Antibody, clone 8G2
      Background InformationPeripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
      Product Information
      FormatCulture Supernatant
      Control
      • Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate
      PresentationUnPurified mouse culture supernatant containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.

      Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

      Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenElectrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE
      Clone8G2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.
      Gene Symbol
      • PRPH
      • PRPH1
      • Peripherin.
      • peripherin
      • NEF4
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41219 # Class-III neuronal intermediate filament protein.
      SIZE: 470 amino acids; 53651 Da
      SIMILARITY: SwissProt: P41219 ## Belongs to the intermediate filament family.
      Molecular Weight57kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size300 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1527 04053252666537