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MABF2122-100UG Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1

Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: PCBP2 UniProt Number: Q15366
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MABF2122-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2122-100UG
      DescriptionAnti-PCBP2 (hnRNP E2) Antibody, clone PC81-1
      Alternate Names
      • Poly(rC)-binding protein 2
      • Alpha-CP2
      • Heterogeneous nuclear ribonucleoprotein E2
      • hnRNP E2
      Background InformationPoly(rC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human. PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied. PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs. It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation. PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation. Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding. Eight different isoforms of PCBP2 have been described that are produced by alternative splicing. (Ref.: Ghanem, LR et al. (2014). Gene Exp. Patterns 14(2); 78-87; Sean, P., et al. (2008). Virology. Virology. 378(2); 243-253).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).

      Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).

      Western Blotting Analysis: 1 µg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
      Biological Information
      ImmunogenHis-tagged full-length human recombinant Poly(rC)-binding protein 2.
      ClonePC 81-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PCBP2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~38 kDa observed; 38.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected PCBP2 (hnRNP E2) in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2122-100UG 04054839563324