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MAB388-100UG Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1

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MAB388-100UG
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MaIHC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB388-100UG
      ReplacesMAB388
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1
      Alternate Names
      • PLP
      Product Information
      FormatPurified
      Control
      • Brain
      PresentationPurified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.

      Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.

      Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.

      Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenMyelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
      EpitopeC-terminus
      ClonePLPC1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
      IsotypeIgG2a
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified for this gene.
      Gene Symbol
      • PLP
      • PMD
      • MMP
      • SPG2
      • PLP/DM20
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P60201 # This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
      SIZE: 277 amino acids; 30,077 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      PTM: Lipoprotein, Palmitate
      DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder of myelin metabolism. It is characterized by early impairment of motor development (during the first three months of life) and, later, by the development of abnormal movements and progressive spastic paraplegia.
      SIMILARITY: Belongs to the myelin proteolipid protein family.
      Molecular Weight23-25 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB388-100UG 04053252360152