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MABF2074-200UL Anti-MuLV TM Antibody, clone 42-114

Anti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a rat monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting.

Anti-MuLV TM Antibody, clone 42-114 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
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MABF2074-200UL
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusIP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2074-200UL
      DescriptionAnti-MuLV TM Antibody, clone 42-114
      Alternate Names
      • Murine leukemia virus
      Background InformationThe murine leukemia virus (MuLV) is a gammaretrovirus that has a positive, single-stranded sense RNA, which replicates via reverse transcription. The envelope of the virus is shown to be covered with glycoprotein spikes. Three major genes produced by the murine leukemia virus are gag, pol, and env. The gag gene codes for the group-specific antigen that is responsible for the production of the viral matrix capsid and nucleoproteins. The pol gene encodes reverse transcriptase, a protease, and an integrase. The reverse transcriptase is used to make complementary DNA by reverse transcribing its own RNA into DNA. The reverse transcriptase in MuLV is able to act as a monomer as opposed to a dimer. The integrase integrates the synthesized viral DNA into the host cell s DNA. The env gene codes for a glycosylated protein that is processed into the two viral envelope proteins, gp70 and p15(E), which aid the virus in selecting and attacking host cells. The gp70 protein has the receptor binding activity of the virus. Retrovirus membrane fusion is catalyzed by the Env, surface (SU) and transmembrane (TM) proteins. The SU protein is involved in receptor binding, while the TM protein contains the necessary elements for membrane fusion. Clone 42-114 is TM-specific and does not recognize G541R mutants. (Ref. Pinter,A., et al. (1982). Virology, 116(2); 499-516. Schneider, WM., et al. (2008). Virology, 371(1); 165-174).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IGM in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MuLV TM, clone 42-114, Cat. No. MABF2074, is a rat monoclonal antibody that detects Murine leukemia virus (MuLV) transmembrane protein and has been tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated MuLV TM (Pinter, A., et. al. (1982). Virology. 116(2):499-516).

      Western Blotting Analysis: A representative lot detected MuLV TM in Western Blotting applications (Schneider, W.M., et. al. (2008). Virology. 371(1):165-74).
      Biological Information
      ImmunogenAKR K36 and AKR SL3 leukemia virus.
      Clone42-114
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 42-114 detects murine leukemia virus in infected cells.
      IsotypeIgMκ
      Species Reactivity
      • Virus
      Species Reactivity NoteMurine Leukemia Virus.
      Antibody TypeMonoclonal Antibody
      Molecular Weight~15 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells infected with murine leukemia virus (MuLVs).

      Western Blotting Analysis: A 1:250 dilution of this antibody detected MuLV TM in HEK293 cells infected with murine leukemia virus (MuLVs).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2074-200UL 04054839448256