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ABE1076 Anti-Misu/NSun2 Antibody

Anti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.

Anti-Misu/NSun2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: NSUN2, SAKI, TRM4 UniProt Number: Q08J23
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ABE1076
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1076
      DescriptionAnti-Misu/NSun2 Antibody
      Alternate Names
      • tRNA (cytosine(34)-C(5))-methyltransferase
      • Myc-induced SUN domain-containing protein
      • Misu
      • NOL1/NOP2/Sun domain family member 2
      • Substrate of AIM1/Aurora kinase B
      • tRNA (cytosine-5-)-methyltransferase
      • tRNA methyltransferase 4 homolog
      • hTrm4
      Background InformationMisu/NSun2, also known as tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein (MISU), NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, or tRNA methyltransferase 4 homolog (hTRM4), and encoded by the gene NSUN2/SAKI/TRM4, is a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Misu/NSun2 methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Functionally Misu/NSun2 acts downstream of Myc to regulate epidermal cell growth and proliferation, and Misu/NSun2-mediated cytosine-5 methylation of vault noncoding RNA helps to determine the processing of them into regulatory small RNAs, and Misu/NSun2 also plays a critical role in gene expression. Interestingly too, Misu/NSun2 is required for proper spindle assembly and chromosome segregation, which is independent of its methyltransferase activity. Misu/NSun2 is localized in the nucleus and nucleolus during rest but during cytokinesis it can be localized in the spindle. Mutations are associated with mental retardation.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human keratinocytes (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human epidermis tissue (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Misu/NSun2.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NSUN2
      • SAKI
      • TRM4
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Misu/NSun2 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1076 04053252972911

      Documentation

      Anti-Misu/NSun2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Misu/NSun2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Misu/NSun2 - Q2420922 Q2420922
      Anti-Misu/NSun2 - 3284332 3284332
      Anti-Misu/NSun2 -2695287 2695287

      References

      Reference overviewPub Med ID
      The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.
      Frye, Michaela and Watt, Fiona M
      Curr. Biol., 16: 971-81 (2006)  2006

      Show Abstract       		16713953 16713953