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MABD399 Anti-Lgr5/GPR49 Antibody, clone 5C8

Anti-Lgr5/GPR49 Antibody, clone 5C8 is an antibody against Lgr5/GPR49 for use in Flow Cytometry, Immunocytochemistry.

Anti-Lgr5/GPR49 Antibody, clone 5C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Gene Symbol: LGR5, GPR49, GPR67, GRP49, HG38 UniProt Number: O75473
MABD399
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABD399
      DescriptionAnti-Lgr5/GPR49 Antibody, clone 5C8
      Alternate Names
      • Leucine-rich repeat-containing G-protein coupled receptor 5
      • G-protein coupled receptor 49
      • G-protein coupled receptor 67
      • G-protein coupled receptor HG38
      • Orphan G protein-coupled receptor HG38
      Background InformationLeucine-rich repeat-containing G-protein coupled receptor 5 (UniProt O75473; also known as G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Orphan G protein-coupled receptor HG38) is encoded by the LGR5 (also known as GPR49, GPR67, GRP49, HG38) gene (Gene ID 8549) in human. Lgr5 is a 7-transmembrane protein related to the receptors for the follicle stimulating hormone (FSH), the luteinizing hormone (LH), and the thyroid-stimulating hormone (TSH). Lgr5 is a Wnt signaling pathway targeted gene expressed in proliferative stem cells in various tissues, including small intestine, colon, stomach, and the hair follicle. The R-spondin (RSPO) family of secreted Wnt signaling agonists are known to bind Lgr5 and the related Lgr4 and Lgr6, and RSPO1 depends on its association wth Lgr4, Lgr5, or Lgr6 for its Wnt signaling enhancing effect. Human Lgr5 is synthesized with a propeptide sequence (a.a. 1-21), the removal of which yields the mature 7-transmembrane (a.a. 562-582 , 594-614, 639-659, 683-703, 723-743, 768-788, 803-823) G-protein-coupled receptor (GPCR) having its N-terminal end (a.a. 22-561) at the extracellular side and its C-terminal end (a.a. 824-907) at the cytoplasmic side. The N-terminal extracellular region contains a leucine-rich repeat (LRR) N-terminal (LRRNT) domain (a.a. 25-66), followed by 16 LRRs (LRR 1 through LLR 16; a.a. 67-446).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Lgr5/GPR49 Antibody, clone 5C8 is an antibody against Lgr5/GPR49 for use in Flow Cytometry, Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 2.0 µg/mL of this antibody detected both transiently and stably expressed human Lgr5 in HEK293T cells (Courtesy of Dr. Wim de Lau, Hubrecht Institute and University Medical Center Utrecht, The Netherlands).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Lgr5/GPR49.
      Clone5C8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LGR5
      • GPR49
      • GPR67
      • GRP49
      • HG38
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~100 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in human Lgr5-expressing stable LS174 (clone 11) cells.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected Lgr5/GPR49 in human Lgr5-expressing stable LS174 (clone 11) cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD399 04055977297362