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MABF1997-100UG Anti-Lap2alpha Antibody, clone 8C10-1H11

Anti-Lap2alpha, clone 8C10-1H11, Cat. No. MABF1997, is a mouse monoclonal antibody that detects Lamina-associated polypeptide 2, isoforms alpha, and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting.

MABF1997-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF1997-100UG
      DescriptionAnti-Lap2alpha Antibody, clone 8C10-1H11
      Alternate Names
      • Lamina-associated polypeptide 2 isoform alpha
      • Thymopoietin isoforms alpha
      • TP alpha
      Background InformationLamina-associated polypeptide 2, isoforms alpha (UniProt: Q61033; also known as Thymopoietin isoform alpha, TP alpha) is encoded by the Tmpo (also known as Lap2) gene (Gene ID: 21917) in murine species. LAP2alpha is a non-membrane bound, oligomeric nucleoplasmic protein that associates directly or indirectly with lamins at specific cell-cycle stages. It is expressed diffusely throughout the nucleus and is involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. It is shown to interact with A-type lamins to control gene expression, transcription, and chromatin organization. The N-terminal part of LAP2alpha contains two structurally independent, non-interacting domains: LEM (LAPEmerin-MAN1)-like domain that binds DNA and a LEM domain that interacts with BANF1. Its C-terminal domain forms a four-stranded coiled coil. Lamin A/C and LAP2alpha complexes predominantly co-occur in euchromatic genomic regions and are shown to be highly mobile. They can bind chromatin in a dynamic manner and are involved in establishing a chromatin environment that is permissive for epigenetic regulation. Loss of LAP2alpha has been linked to increased proliferation of tissue progenitor cells in epidermis, colon, and the hematopoietic system and delayed skeletal muscle differentiation. (Res.: Gesson, K et al., (2016). Genome Res. 26(4);462-473).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Lap2alpha, clone 8C10-1H11, Cat. No. MABF1997, is a mouse monoclonal antibody that detects Lamina-associated polypeptide 2, isoforms alpha, and has been tested for use in Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).

      Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region.
      Epitopecytoplasmic domain
      Clone8C10-1H11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tmpo
      • Lap2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~75 kDa observed; 75.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Knockout (KO) vs. wild-type (WT) mouse fibroblast cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Lamina-associated polypeptide 2, isoforms alpha (LAP2alpha) in wild-type, but not in Knockout (KO) mouse fibroblast cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1997-100UG 04054839577116

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