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MABF294 Anti-LAG3 Antibody, clone 8C7.1

This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Anti-LAG3 Antibody, clone 8C7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: LAG3, FDC UniProt Number: P18627
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MABF294
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABF294
      DescriptionAnti-LAG3 Antibody, clone 8C7.1
      Alternate Names
      • Lymphocyte activation gene 3 protein
      • LAG-3
      • Protein FDC
      • CD223
      • LAG3
      Background InformationLymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
      Biological Information
      Immunogen Mouse Fc-tagged recombinant human LAG3 extracellular fragment.
      Epitopeextracellular domain
      Clone8C7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAG3
      • FDC
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~60 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NK-92®c ell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92® cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF294 04055977274936