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AB1832P Anti-Interleukin-1β Antibody

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AB1832P
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RELISA, WB, NEUTRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1832P
      Replaces04-462; MABF22
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Interleukin-1β Antibody
      Alternate Names
      • IL-1beta
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified Immunoglobulin. Lyophilized from 0.5X PBS pH 7.4, no preservatives. Reconstitute in sterile distilled water.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Interleukin-1β Antibody is an antibody against Interleukin-1β for use in ELISA, WB, NEUT.
      Key Applications
      • ELISA
      • Western Blotting
      • Neutralizing
      Application NotesELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of

      0.2-0.4 ng/well when rat IL-1(is used to coat the plate.

      Western blot: 0.1-0.2 μg/mL, this dilution should offer detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.

      Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 μg/mL of antibody is required.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenHighly pure (>98%) recombinant rat IL-1beta.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReacts with rat Interleukin-1beta (IL-1beta).
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
      Gene Symbol
      • IL1B
      • IL1F2
      • pro-interleukin-1-beta
      • IL1-BETA
      • Catabolin
      • IL-1
      • IL-1B
      • catabolin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01584 # Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
      SIZE: 269 amino acids; 30748 Da
      SUBUNIT: Monomer.
      SUBCELLULAR LOCATION: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
      DOMAIN: SwissProt: P01584 The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
      SIMILARITY: Belongs to the IL-1 family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB1832P 04053252324512