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MABS1920 Anti-IRF-9 Antibody, clone 6F1-H5

Anti-IRF-9, clone 6F1-H5, Cat. No. MABS1920, is a mouse monoclonal antibody that detects Interferon regulatory factor 9 (IRF-9). It has been tested for use in chromatin Immunoprecipitation and Western Blotting.

Anti-IRF-9 Antibody, clone 6F1-H5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Irf9, Isgf3g UniProt Number: Q61179
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MABS1920
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1920
      DescriptionAnti-IRF-9 Antibody, clone 6F1-H5
      Alternate Names
      • Interferon regulatory factor 9
      • IFN-alpha-responsive transcription factor subunit
      • ISGF3 p48 subunit
      • Interferon-stimulated gene factor 3 gamma
      • ISGF-3 gamma
      • Transcriptional regulator ISGF3 subunit gamma
      Background InformationInterferon regulatory factor 9 (UniProt: Q61179; also known as IRF-9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma) is encoded by the Irf9 (also known as Isgf3g) gene (Gene ID: 16391) in murine species. IRF-9 is a transcription factor that mediates signaling by interferon-alpha and beta. It contains 1 IRF tryptophan pentad repeat DNA-binding domain Following binding of these interferons to cell surface receptors, JAK kinases (Tyk2 ad JAK1) are activated that lead to tyrosine phosphorylation of STAT1 and STAT2. IRF-9 then associates with phosphorylated STAT1:STAT2 dimer to form the interferon-stimulated gene factor 3 (ISFG3) complex that translocates to the nucleus and binds to the interferon stimulated response element (ISRE) to activate the transcription of interferon-stimulated genes. In mice, ablation of IRF-9 is shown to block the proliferation and migration of vascular smooth muscle cells (VSMC) and attenuates intimal thickening in response to injury. On the other hand, IRF-9 gain-of-function is reported to promote VSMC proliferation and migration that can aggravates arterial narrowing. (Ref.: Zhang, SM et al (2015). Nat Commun. 6:6882).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-IRF-9, clone 6F1-H5, Cat. No. MABS1920, is a mouse monoclonal antibody that detects Interferon regulatory factor 9 (IRF-9). It has been tested for use in chromatin Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation: Chromatin Immunoprecipitation was performed on a representative lot with wild -type or IRF9 knock-out bone marrow derived marcrophages (uninduced or treated with IFN beta). (Data courtesy of Prof. Thomas Decker, Ph.D., University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged full length recombinant longer version (465 amino acids) of murine Interferon regulatory factor 9.
      EpitopeN-terminus
      Clone6F1-H5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6F1-H5 detects interfereon regulatory factor 9 (IRF-9) of 51 kDa in murine species. May also detect shorter variants of IRF-9.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Does not react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Irf9
      • Isgf3g
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~51 kDa observed. 44.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse spleen tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected IRF-9 in 10 µg of mouse spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1920 04054839224164