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ABN903-25UG Anti-Huntingtin

Anti-Huntingtin, Cat. No. ABN903, is a highly specific rabbit polyclonal antibody that targets Huntington Disease Protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Huntingtin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: HTT, HD, IT15 UniProt Number: P42858
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ABN903-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN903-25UG
      DescriptionAnti-Huntingtin
      Alternate Names
      • Huntington disease protein
      • HD protein
      Background InformationHuntingtin (UniProt: P42858; also known as Huntington disease protein, HD protein) is encoded by the HTT (also known as HD, IT15) gene (Gene ID: 3064) in human. The protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. It is expressed in the brain and is mainly found in the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Huntingtin is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. It plays a role in microtubule-mediated transport and vesicle function. Huntingtin protein contains a nuclear export signal (aa 2395-2404) and 5 HEAT (Huntington, Elongation Factor 3, PR65/A, TOR) domains. Huntingtin protein is cleaved by apopain downstream of the polyglutamine stretch and the resulting N-terminal fragment is cytotoxic and provokes apoptosis. Phosphorylation at Serine 1179 and 1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Mutations in HTT gene cause Huntington disease that is characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Wild-type huntingtin and anti-huntingtin antibody reduces aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of Huntington disease.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Huntingtin, Cat. No. ABN903, is a highly specific rabbit polyclonal antibody that targets Huntington Disease Protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Huntingtin  in mouse brain tissue lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 180 amino acids from the N-terminal region of human Huntington disease protein .
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Huntington disease protein in human and mouse brain. It targets an epitope within 180 amino acids from the N-terminal region.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • HTT
      • HD
      • IT15
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight347.60 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex and human huntington's diseased brain tissues. Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody detected Huntingtin  in human cerebral cortex and human Huntington's diseased brain tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN903-25UG 04054839543647