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07-594 Anti-Histone H2A.Z Antibody

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07-594
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-594
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2A.Z Antibody
      References
      Product Information
      FormatSerum
      Presentation0.05% sodium azide before the addition of glycerol to 30%
      Quality LevelMQ100
      Applications
      ApplicationDetect Histone H2A.Z also known as H2AZ histone with Anti-Histone H2A.Z Antibody (Rabbit Polyclonal Antibody), that has been demonstrated to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide containing the sequence SLIGKKGQQ, corres-ponding to the C-terminus of human histone H2A.Z
      HostRabbit
      SpecificityC-terminus of histone H2A.Z
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteBroad species cross-reactivity is expected
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
      Gene Symbol
      • H2AFZ
      • H2A/z
      • H2A.Z
      • MGC117173
      • H2AZ
      • H2A.z
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P0C0S5 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
      SIZE: 128 amino acids; 13553 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. & Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity). & Not phosphorylated (By similarity).
      SIMILARITY: SwissProt: P0C0S5 ## Belongs to the histone H2A family.
      Molecular Weight16kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from HeLa cells and recombinant Histone H2A.Z (Catalog #14-597), no cross-reactivity with Histone H2A
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-594 04053252405662

      Documentation

      Anti-Histone H2A.Z Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H2A.Z Antibody Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A.Z (rabbit antiserum) - 2455725 2455725
      Anti-Histone H2A.Z (rabbit antiserum) Q2912987
      Anti-Histone H2A.Z (rabbit antiserum) 3046749
      Anti-Histone H2A.Z (rabbit antiserum) 2865454
      Anti-Histone H2A.Z (rabbit antiserum) 2944559
      Anti-Histone H2A.Z (rabbit antiserum) - 2275603 2275603
      Anti-Histone H2A.Z - 26980 26980
      Anti-Histone H2A.Z - 3488178 3488178
      Anti-Histone H2A.Z - 3724732 3724732
      Anti-Histone H2A.Z - 3916156 3916156

      References

      Reference overviewApplicationPub Med ID
      Genome-wide targeting of the epigenetic regulatory protein CTCF to gene promoters by the transcription factor TFII-I.
      Peña-Hernández, R; Marques, M; Hilmi, K; Zhao, T; Saad, A; Alaoui-Jamali, MA; del Rincon, SV; Ashworth, T; Roy, AL; Emerson, BM; Witcher, M
      Proceedings of the National Academy of Sciences of the United States of America  112  E677-86  2015

      Show Abstract
      25646466 25646466
      Dynamics of DOT1L localization and H3K79 methylation during meiotic prophase I in mouse spermatocytes.
      Ontoso, D; Kauppi, L; Keeney, S; San-Segundo, PA
      Chromosoma  123  147-64  2014

      Show Abstract
      Immunofluorescence24105599 24105599
      Phosphorylation and arginine methylation mark histone H2A prior to deposition during Xenopus laevis development.
      Wang, WL; Anderson, LC; Nicklay, JJ; Chen, H; Gamble, MJ; Shabanowitz, J; Hunt, DF; Shechter, D
      Epigenetics & chromatin  7  22  2014

      Show Abstract
      Immunoblotting (Western)25302076 25302076
      Histone H3.3 and its proteolytically processed form drive a cellular senescence programme.
      Duarte, LF; Young, AR; Wang, Z; Wu, HA; Panda, T; Kou, Y; Kapoor, A; Hasson, D; Mills, NR; Ma'ayan, A; Narita, M; Bernstein, E
      Nature communications  5  5210  2014

      Show Abstract
      25394905 25394905
      Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis.
      Dowdle, JA; Mehta, M; Kass, EM; Vuong, BQ; Inagaki, A; Egli, D; Jasin, M; Keeney, S
      PLoS genetics  9  e1003945  2013

      Show Abstract
      24244200 24244200
      Molecular cloaking of H2A.Z on mortal DNA chromosomes during nonrandom segregation.
      Yang Hoon Huh,James L Sherley
      Stem cells (Dayton, Ohio)  29  2011

      Show Abstract
      21905168 21905168
      Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.
      Ma, MK; Heath, C; Hair, A; West, AG
      PLoS genetics  7  e1002175  2011

      Show Abstract
      21811414 21811414
      Synergism between DNA methylation and macroH2A1 occupancy in epigenetic silencing of the tumor suppressor gene p16(CDKN2A).
      Barzily-Rokni, M; Friedman, N; Ron-Bigger, S; Isaac, S; Michlin, D; Eden, A
      Nucleic acids research  39  1326-35  2011

      Show Abstract
      21030442 21030442
      Mapping and analysis of chromatin state dynamics in nine human cell types.
      Ernst, J; Kheradpour, P; Mikkelsen, TS; Shoresh, N; Ward, LD; Epstein, CB; Zhang, X; Wang, L; Issner, R; Coyne, M; Ku, M; Durham, T; Kellis, M; Bernstein, BE
      Nature  473  43-9  2011

      Show Abstract
      21441907 21441907
      Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice.
      Nashun, B; Yukawa, M; Liu, H; Akiyama, T; Aoki, F
      Development (Cambridge, England)  137  3785-94  2010

      Show Abstract
      Western Blotting20943707 20943707

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies