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OB05 Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-His6

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OB05
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
AMMonoclonal Antibody

Pricing & Availability

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OB05-100UG
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      Description
      OverviewSpecifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.
      Catalogue NumberOB05
      Brand Family Calbiochem®
      SynonymsAnti-His6
      Application Data
      Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence.

      Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.
      References
      ReferencesZentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
      Gu, J., et al. 1994. BioTechniques 17, 257.
      Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
      Sisk, W.P., et al. 1994. J. Virol. 68, 766.
      Lu, T., et al. 1993. Anal. Biochem. 213, 318.
      Garner, J., et al. 1992. Cell 69, 833.
      Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.
      Product Information
      DeclarationManufactured by Dianova, GmbH, Germany.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlProteins containing a His•Tag® sequence
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesELISA (0.1-0.4 µg/ml)
      Immunoblotting (1-4 µg/ml)
      Immunofluorescence (1-4 µg/ml)
      Immunoprecipitation (4 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein containing a His•Tag® sequence
      EpitopeHHHHHH
      Clone13/45/31/2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OB05-100UG 04055977210057

      Documentation

      Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) Certificates of Analysis

      TitleLot Number
      OB05

      References

      Reference overview
      Zentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
      Gu, J., et al. 1994. BioTechniques 17, 257.
      Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
      Sisk, W.P., et al. 1994. J. Virol. 68, 766.
      Lu, T., et al. 1993. Anal. Biochem. 213, 318.
      Garner, J., et al. 1992. Cell 69, 833.
      Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-January-2009 JSW
      SynonymsAnti-His6
      ApplicationELISA (0.1-0.4 µg/ml)
      Immunoblotting (1-4 µg/ml)
      Immunofluorescence (1-4 µg/ml)
      Immunoprecipitation (4 µg/ml)
      Application Data
      Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence.

      Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag® sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
      BackgroundIdentification of proteins for which there does not exist antibodies has traditionally been overcome by the use of epitope tagging systems in which expression vectors containing an open reading frame encoding a small fragment of a specific protein (for which an antibody exists) together with multiple cloning sites are used to express foreign genes. Several different epitope tagging systems of this type currently in use include maltose-binding-protein (MBP), glutathione S-transferase (GST), TrpE, and myc. Tags are generally placed at either the N-or C-terminus of the protein as epitope tags placed within a coding sequence of a target protein increases the possibility of interference with the function of the protein and also decreases ability of tag-specific antibody to detect epitope tagged sequence buried by native folding of the target protein. A second type of epitope tagging involves the addition of multiple histidine residues, usually six residues, at either the N- or C-terminus of the target protein. Expressed proteins with such a tag may then be purified by binding to divalent metal ion columns, most commonly to chelated-Ni2+ columns using a technique known as IMAC or immobilized metal ion chromatography. This type of epitope tagging is particularly suited for the purification of recombinant proteins from E. coli expression systems. Analysis of histidine-tagged proteins by immunoblotting or immunofluorescence may also be done using an antibody to the histidine tag itself, thereby eliminating the need for an antibody specific for the protein in question.
      HostMouse
      Immunogena recombinant protein containing a His•Tag® sequence
      EpitopeHHHHHH
      Clone13/45/31/2
      IsotypeIgG₁
      Speciesall His•Tag® proteins
      Positive controlProteins containing a His•Tag® sequence
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesZentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
      Gu, J., et al. 1994. BioTechniques 17, 257.
      Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
      Sisk, W.P., et al. 1994. J. Virol. 68, 766.
      Lu, T., et al. 1993. Anal. Biochem. 213, 318.
      Garner, J., et al. 1992. Cell 69, 833.
      Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.