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MAB2600 Anti-Glypican-1 Antibody, clone 4D1

Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.

Anti-Glypican-1 Antibody, clone 4D1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: FLJ38078, glypican UniProt Number: P35052
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MAB2600
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2600
      DescriptionAnti-Glypican-1 Antibody, clone 4D1
      Alternate Names
      • glypican 1
      • glypican proteoglycan 1
      Background InformationHeparan sulfate proteoglycans (HSPGs) can be divided into general categories of
      cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
      Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).
      References
      Product Information
      FormatPurified
      Control
      • PC-12 cell lysate.
      PresentationPurified in 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.
      Biological Information
      ImmunogenFull length recombinant protein.
      EpitopeFull Length
      Clone4D1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB2600 recognizes glypican-1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq].
      Gene Symbol
      • FLJ38078
      • glypican
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Cell surface proteoglycan that bears heparan sulfate.

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side.

      Secreted glypican-1: Secreted › extracellular space.

      PTM: This cell-associated glypican is further processed to give rise to a medium-released species.

      SEQUENCE SIMILARITIES: Belongs to the glypican family.
      Molecular WeightRecognizes Glypican-1 at ~ 59 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2600 04053252399770

      Documentation

      Anti-Glypican-1 Antibody, clone 4D1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glypican-1 Antibody, clone 4D1 Certificates of Analysis

      TitleLot Number
      Anti-Glypican-1, clone 4D1 2477953
      Anti-Glypican-1, clone 4D1 - 2172757 2172757
      Anti-Glypican-1, clone 4D1 - 2197302 2197302
      Anti-Glypican-1, clone 4D1 - 2210384 2210384
      Anti-Glypican-1, clone 4D1 - 2280472 2280472
      Anti-Glypican-1, clone 4D1 - 2303965 2303965
      Anti-Glypican-1, clone 4D1 - 2492818 2492818
      Anti-Glypican-1, clone 4D1 - 2533154 2533154
      Anti-Glypican-1, clone 4D1 - 3167059 3167059
      Anti-Glypican-1, clone 4D1 - 3732305 3732305
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