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AB10541 Anti-Galectin-3 Antibody

This Anti-Galectin-3 Antibody is validated for use in WB for the detection of Galectin-3.

Anti-Galectin-3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: LGALS3, MAC2 UniProt Number: P17931
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AB10541
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Rhesus MacaqueWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10541
      DescriptionAnti-Galectin-3 Antibody
      Alternate Names
      • Galectin-3
      • Gal-3
      • 35 kDa lectin
      • Carbohydrate-binding protein 35
      • CBP 35
      • Galactose-specific lectin 3
      • Galactoside-binding protein
      • GALBP
      • IgE-binding protein
      • L-31
      • Laminin-binding protein
      • Lectin L-29
      • Mac-2 antigen
      Background InformationGalectin-3 belongs to the galectin gene family, which is defined by the specificity of a carbohydrate recognition domain (CRD) for Gal/Lac. Galectin-3 expression is usually elevated in a wide range of neoplastic cell types. The galectin CRD consists of 135 amino acids. Galectin-3 is known to play a role in tumor development, apoptosis, and cell cycle progression.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • MG-63 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Galectin-3 Antibody is validated for use in WB for the detection of Galectin-3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the Pro64 mutation domain of human Galectin-3.
      EpitopePro64 mutation domain
      HostRabbit
      SpecificityThis antibody recognizes the Pro64 mutation domain of Galectin-3. It is equally specific for wild-type or mutant His64 Galectin-3.
      Species Reactivity
      • Human
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
      Gene Symbol
      • LGALS3
      • MAC2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

      SUBUNIT STRUCTURE: Probably forms homo- or heterodimers. Interacts with DMBT. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface.

      TISSUE SPECIFICITY: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
      Molecular Weight~33 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MG-63 cell lysate.

      Western Blot Analysis: A 1:250 dilution of this antibody detected Galectin-3 in 10 µg of MG-63 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB10541 04053252634642