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MABF3170-25UG Anti-GTPase HfIX Antibody, clone 49

Anti-GTPase HfIX, clone 49, Cat. No. MABF3170, is a mouse monoclonal antibody that detects GTPase HfIX and is tested for use in Dot Blot, ELISA, and Immunoprecipitation.

MABF3170-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3170-25UG
      DescriptionAnti-GTPase HfIX Antibody, clone 49
      Alternate Names
      • High frequency of lysogenization X GTPase
      Background InformationGTPase HflX (UniProt: O33230; also known as GTP-binding protein HflX) is encoded by the hflX (also known as Rv2725c) gene (Gene ID: 888241) in Mycobacterium tuberculosis. High frequency of lysogenization X (HflX) protein belongs to the superfamily of the Obg-HflX like ucpGTPases class of translation factors that is highly conserved in the entire prokaryotic kingdom. It is involved in the stress response of bacteria and participates in protein translation, likely by providing energy for protein synthesis, or by facilitating the recycling of factors involved in translation. HflX is a ribosome-binding protein that binds to the E-site of 70S bacterial ribosomes and induces split into 50S/30S ribosomal subunits upon GTP hydrolysis. HflX produced by slow-growing Mycobacterium bovis (M. bovis) is reported to play a critical role in the pathogen s transition to a nonreplicating, drug-tolerant state in response to hypoxia. On the other hand, HflX-deficient M. bovis (knockout) replicate at a markedly faster rate in the microaerophilic phase of a hypoxia model, which results in premature entry into dormancy. The absence of HflX also results in a significant accumulation of 70S ribosomes upon erythromycin exposure, and deletion of its N-terminal or the C-terminal domain abrogates ribosome splitting and abolishes its ability to mediate antibiotic tolerance. HflX is overexpressed in M. bovis exposed to the hostile lysosomal environment of macrophages. Under normoxia culture conditions it does not play any essential role in M. bovis, but under hypoxic state it modulates translational activity and controls Mycobacterium growth rate. (Ref.: Ngan, JYG., et al. (2021) Proc. Natl. Acad. Sci. USA. 118(12); e2006717118; Rudra, P., et al., (2020). Proc. Natl. Acad. Sci. USA. 117(1); 629-634).)
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GTPase HfIX, clone 49, Cat. No. MABF3170, is a mouse monoclonal antibody that detects GTPase HfIX and is tested for use in Dot Blot, ELISA, and Immunoprecipitation.
      Key Applications
      • Dot Blot
      • ELISA
      • Immunoprecipitation
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected HfIX in ELISA application (Ngan, J.Y.G., et al. (2021). Proc Natl Acad Sci USA. 118(12):e2006717118).

      ELISA Analysis: Various dilutions of this antibody detected recombinant GTPase HfIX protein.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated GTPase HfIX in Immunoprecipitation application (Ngan, J.Y.G., et al. (2021). Proc Natl Acad Sci USA. 118(12):e2006717118).

      Dot Blot: A representative lot detected HfIX in Dot Blot applications (Ngan, J.Y.G., et al. (2021). Proc Natl Acad Sci USA. 118(12):e2006717118).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant GTPase HflX from Mycobacterium tuberculosis.
      EpitopeUnknown
      Clone49
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 49 is a mouse monoclonal antibody that detects GTPase High frequency of lysogenization X (HflX).
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      Species Reactivity NoteMycobacterium sp.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • hFlX
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight53.33 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot Analysis with His-tagged, recombinant GTPase HfLX from Mycobacterium tuberculosis.

      Dot Blot Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant GTPase HflX from mycobacterium tuberculosis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3170-25UG 04065270453617