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ABN27 Anti-GRIP-1 Antibody, CT

Detect GRIP-1 using this Anti-GRIP-1 Antibody, C-terminus validated for use in WB, IP, IH(P).

Anti-GRIP-1 Antibody, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: GRIP, GRIP-1, GRIP1, CT UniProt Number: P97879
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ABN27
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, ChpWB, IP, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN27
      Replaces06-986
      DescriptionAnti-GRIP-1 Antibody, CT
      Alternate Names
      • glutamate receptor interacting protein 1
      • glutamate receptor-interacting protein 1
      Background InformationGlutamate receptor interacting protein-1 (GRIP-1) is a multi-PDZ domain scaffold protein and a member of the steroid receptor coactivator (SRC) family of proteins. GRIP-1 interacts and maneuvers kinesin heavy chains to dendrites by acting as the power source for AMPA receptors. During initial stages of embryonic development, GRIP-1 is essential for typical cell-matrix interactions.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat brain cytosol tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect GRIP-1 using this Anti-GRIP-1 Antibody, C-terminus validated for use in WB, IP, IH(P).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated GRIP-1 from rat brain cytosol tissue lysate.

      Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected GRIP-1 in rat brain cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of rat GRIP-1.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes GRIP-1 at the C-terminus.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with rat. Predicted to react with human, mouse, and chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBinds to the liprin-alpha SVD2 family of proteins to mediate synaptic targeting of AMPA receptors; may play a role in presynaptic and postsynaptic maturation [RGD].
      Gene Symbol
      • GRIP
      • GRIP-1
      • GRIP1
      • CT
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.

      SUBUNIT STRUCTURE: Interacts with EFNB1, EPHA7, EPHB2, EFNB3, KIF5A, KIF5C, KIF5B and the C-terminal tail of PRLHR. Forms a ternary complex with GRIA2 and CSPG4 (By similarity). Can form homomultimers or heteromultimers with GRIP2. Interacts with GRIA2, GRIA3, GRIPAP1/GRASP1, PPFIA1, PPFIA4, FRAS1, PLCD4, PTPRF and liprins-alpha. Interacts with SLC30A9 (By similarity).

      SUBCELLULAR LOCATION: Cytoplasm. Endomembrane system; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Endoplasmic reticulum membrane; Peripheral membrane protein (Potential). Note: Membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.

      TISSUE SPECIFICTY: Expressed in brain, testis and retina. In brain highly expressed in the olfactory bulb, cortex and hyppocampus and lower level in thalamus, cerebellum and spinal cord. In brain it is found in the perikaryon, dendrites, dendritic shafts, dendritic spines and, excitatory and inhibitory synapses of neurons. In retina, it is most abundant in the plexiform layers than in perikarya.

      DEVELOPMENTAL STAGE: Detected in early embryonic stage as early as E15, gradually increased throughout early development, peaked at approximately between postnatal days P6 and P8, then slightly decreased and remained relatively stable in the adult.

      DOMAIN: PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with PRLHR (By similarity). PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1.

      SEQUENCE SIMILARITIES: Contains 7 PDZ (DHR) domains.
      Molecular Weight~121 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain cytosol tissue lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected GRIP-1 on 10 µg of rat brain cytosol tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN27 04053252749513

      Documentation

      Anti-GRIP-1 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GRIP-1 Antibody, CT Certificates of Analysis

      TitleLot Number
      Anti-GRIP-1, C-terminus - 2450774 2450774
      Anti-GRIP-1, C-terminus - 2006941 2006941
      Anti-GRIP-1, C-terminus - 2110523 2110523
      Anti-GRIP-1, C-terminus - 2266765 2266765
      Anti-GRIP-1, C-terminus - 2844193 2844193
      Anti-GRIP-1, C-terminus - 2857475 2857475
      Anti-GRIP-1, C-terminus - 3160210 3160210
      Anti-GRIP-1, C-terminus - 3482835 3482835
      Anti-GRIP-1, C-terminus - NRG1843492 NRG1843492
      Anti-GRIP-1, C-terminus -2555297 2555297
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