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MABS1135 Anti-GNAQ Antibody, clone 8A11.1

Anti-GNAQ Antibody, clone 8A11.1 is an antibody against GNAQ for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-GNAQ Antibody, clone 8A11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: G-proteins Gene Symbol: GNAQ, GAQ, SWS UniProt Number: P50148
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MABS1135
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1135
      DescriptionAnti-GNAQ Antibody, clone 8A11.1
      Alternate Names
      • Guanine nucleotide-binding protein G(q) subunit alpha
      • Guanine nucleotide-binding protein alpha-q
      Background InformationGuanine nucleotide-binding protein G(q) subunit alpha (UniProt P50148; also known as Guanine nucleotide-binding protein alpha-q) is encoded by the GNAQ (also known as GAQ, SWS) gene (Gene ID 2776) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (α), beta (β), and gamma (γ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyzes the GDP-to-GTP exchange of the Gα subunit, causing a dissociation of the Gβγ dimer from the now GTP-bound Gα subunit. The Gα subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gβγ and re-starts the cycle. There exist at least 20 different Gα subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Four different genes code for Gq alpha subunits, GNAQ, GNA11, GNA14, and GNA15. Gq proteins mediate a variety of cellular signaling events by activating phospholipase C (PLC). Gq-coupled GPCRs include α1-adrenoreceptor, angiotensin II receptor type 1, calcitonin receptor, muscarinic receptors (M1, M3, and M5), histamine H1 receptor, Serotonin 5-HT2 receptors, vasopressin type 1 receptors 1A and 1B.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GNAQ Antibody, clone 8A11.1 is an antibody against GNAQ for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GNAQ in rat testis, human prostate, human small intestine, and human prostate cancer tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment of human GNAQ.
      EpitopeInternal.
      Clone8A11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8A11.1 was raised against a human Gqα (GNAQ) recombinant fragment. Clone 8A11.1 reactivity toward Gq/11α (GNA11), Gq/14α (GNA14), and Gq/15α (GNA15) has not been tested.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GNAQ
      • GAQ
      • SWS
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~40 kDa observed. 42.14 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human testis tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected GNAQ in 10 µg of human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1135 04055977333589