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MABE2022-25UG Anti-GINS2/PSF2 Antibody, clone 78C

Anti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABE2022-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE2022-25UG
      DescriptionAnti-GINS2/PSF2 Antibody, clone 78C
      Alternate Names
      • DNA replication complex GINS protein PSF2
      • GINS complex subunit 2
      Background InformationDNA replication complex GINS protein PSF2 (UniProt: Q9Y248; also known as GINS complex subunit 2) is encoded by the GINS2 (also known as PSF2, CGI-122, DC5, HSPC037) gene (Gene ID: 51659) in human. GINS/PSF2 is a component of the GINS complex, which is a heterotetramer of GINS 1, 2, 3, and 4 that are assembled with 1:1:1:1 stoichiometry. Each of these four subunits is essential for proper completion of DNA replication initiation and elongation. This complex plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex binds preferentially to single-stranded DNA. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein Cdc45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. GINS2 gene is reported to be frequently up-regulated in the cancer cells. Transfection of ETK-1 and HuH28 cells with PSF2 siRNA is shown to reduce the amount of transcript and suppress cell growth, indicating the important role of GINS2/PF2 in development of cholangiocarcinoma. (Ref.: Aparicio, T., et al. (2009). Nucl. Acids Res. 37(7); 2087-2095; Boskovic, J., et al. (2007). EMBO Rep. 8(7); 678-684; Obama, K., et al. (2005). Oncol. Rep. 14(3):701-706).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

      Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant human GINS2/PSF2 expressed in E. coli.
      EpitopeUnknown
      Clone78C
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 78C is a mouse monoclonal antibody that detects human DNA replication complex GINS protein PSF2 (GINS2/PSF2).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GINS2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight21.43 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE2022-25UG 04065268528914