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MABT1551-100UG Anti-Filaggrin Antibody, clone AHF7

Anti-Filaggrin, clone AHF7, Cat. No. MABT1551, is a mouse monoclonal antibody that detects Filaggrin and is tested for use in Immunohistochemistry (Paraffin), Western Blotting, Electron Microscopy, ELISA, Immunofluorescence, and Immunohistochemistry.

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100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1551-100UG
      DescriptionAnti-Filaggrin Antibody, clone AHF7
      Alternate Names
      • FLG
      Background InformationFilaggrin (UniProt: P20930; also known as FLG) is encoded by the FLG gene (Gene ID: 2312) in human. Filaggrin, a calcium-binding protein, is synthesized as a large, insoluble, highly phosphorylated precursor with several tandem copies of 324 amino acids in cytoplasmic granules. During terminal differentiation it undergoes dephosphorylation and proteolytic cleavage to remove linker peptides and produce functional basic filaggrin units. It is widely expressed and in skin tissue it is detected mainly in stratum granulosum of the epidermis. During terminal differentiation of mammalian epidermis, it aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments. This creates the intracellular fibrous matrix of the cornified cells. Deimination of arginine to citrulline by peptidylarginine deiminase is reported to result in reduction in its affinity for cytokeratins. The N-terminal region of the mature filaggrin is shown to be heterogenous and is blocked by the formation of pyroglutamate. Clone AHF7 detects only the mature form of filaggrin and does not detect profilaggrin. It exhibits reactivity in various immunochemical applications. (Ref.: Simon, M., et al. (1996). Biochem. J. 317(pt 1); 173 177; Simon, M., et al. (1995). Clin. Exp. Immunol. 100(1); 90-98; Simon, M., et al. (1995). J. Invest. Dermatol. 105(3); 432-437).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS with 0.1% sodium azide with 50% glycerol.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Filaggrin, clone AHF7, Cat. No. MABT1551, is a mouse monoclonal antibody that detects Filaggrin and is tested for use in Immunohistochemistry (Paraffin), Western Blotting, Electron Microscopy, ELISA, Immunofluorescence, and Immunohistochemistry.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Electron Microscopy
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected Filaggrin in Immunohistochemistry application. (Simon, M., et al. (1995). Clin Exp Immunol. 100(1):90-8; Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Girbal-Neuhauser, E., et al. (1997). Mol Med. 3(2):145-56; Guy, R., et al. (1998). Dermatology. 196(1):32-7; Guy, R., et al. (1998). J Invest Dermatol. 110(4):410-5; Downie, M.M.T., et al. (2002). Br J Dermatol. 147(5):869-78; Chavanas, S., et al. (2006). J Dermatol Sci. 44(2):63-72).

      Immunofluorescence Analysis: A representative lot detected Filaggrin in Immunfluorescence application. (Simon, M., et al. (1995). Clin Exp Immunol. 100(1):90-8; Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Girbal-Neuhauser, E., et al. (1997). Mol Med. 3(2):145-56; Chavanas, S., et al. (2006). J Dermatol Sci. 44(2):63-72).

      ELISA Analysis: A representative lot detected Filaggrin in ELISA application. (Simon, M., et al. (1996). Biochem J. 317(Pt1)(Pt1):173-7).

      Electron Microscopy: A representative lot detected Filaggrin in Electron Microscopy application. (Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7).

      Western Blotting Analysis: A representative lot detected Filaggrin in Western Blotting application. (Sebbag, M., et al. (1995). J Clin Invest. 95(6):2672-9; Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Simon, M., et al. (1996). Biochem J. 317(Pt1)(Pt1):173-7; Girbal-Neuhauser, E., et al. (1997). Mol Med. 3(2):145-56).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenPartially purified Filaggrin from human breast skin tissue.
      EpitopeUnknown
      CloneAHF7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AHF7 is a mouse monoclonal antibody that detects the mature form of Filaggrin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLG
      Purification MethodDEAE
      UniProt Number
      Molecular Weight435.17 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in Human skin tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Filaggrin in Human skin tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1551-100UG 04065271270046