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MAB1926-I-25UL Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11

This mouse monoclonal Anti-Fibronectin, cell binding domain, clone P1H11 Antibody, Cat. No. MAB1926-I-25UL is validated for use in Western Blotting Immunohistochemistry (Paraffin) and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: FN1, FN UniProt Number: P02751
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MAB1926-I-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Porcine, HWB, ICC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1926-I-25UL
      DescriptionAnti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11
      Alternate Names
      • FN
      • Cold-insoluble globulin
      • CIG
      Background InformationFibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human. Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts. Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region. Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types. Cellular fibronectin is deposited as fibrils in the extracellular matrix. Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3. The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD). Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Fibronectin, cell binding domain, clone P1H11 Antibody, Cat. No. MAB1926-I-25UL is validated for use in Western Blotting Immunohistochemistry (Paraffin) and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Fibronectin in human kidney and human cerebellum tissues.

      Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2010). Am J Physiol Renal Physiol. 299(1):F91-8).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Mardilovich, A., et. al. (2006). Langmuir. 22(7):3259-64).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Hofanson, D.M., et. al. (2010). Biomaterials. 31(26):6730-7).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Waterman, R.S., et. al. (2010). PLoS One. 5(4):e10088).

      Immunohistochemistry Analysis: A representative lot detected Fibronectin in Immunohistochemistry applications (Carlos, C.P., et. al. (2014). PLoS One. 9(7):e103660).

      Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2008). Am J Physiol Renal Physiol. 295(4):F1071-81).
      Biological Information
      ImmunogenHuman Plasma Fibronectin.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone P1H11 detects fibronectin in human.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Porcine
      • Human
      Species Reactivity NoteHuman, Mouse, Porcine.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • FN1
      • FN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight270 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human thymus tissue lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected Fibronectin in 10 µg of h uman thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1926-I-25UL 04054839448010