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MABE1922-25UL Anti-Exportin-5 Antibody, clone EX5 4E1

Anti-Exportin-5, clone EX5 4E1, Cat. No. MABE1922, is a rat monoclonal antibody that detects Exportin-5 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

MABE1922-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1922-25UL
      DescriptionAnti-Exportin-5 Antibody, clone EX5 4E1
      Alternate Names
      • Exp5:Ran-binding protein 21
      Background InformationExportin-5 (UniProt: Q9HAV4; also known as Exp5, Ran-binding protein 21) is encoded by the XPO5 (also known as KIAA1291, RANBP21) gene (Gene ID: 57510) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Exportin-5 mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). Its expression has been reported in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Exportin-5 binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP results in disassembly of the complex and release of the cargo from the export receptor. Exportin-5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. Exportin-5 is shown to be a pre-miRNA-specific export carrier and in this regard, it is reported to contribute to the development and/or progression of malignant melanoma by influencing miRNA transport and maturation. High levels of Exporotin-5 in melanoma cells leads to their enhanced survival, proliferation and metastasis and supports the aggressiveness of melanoma. (Ref.: Ott, CA., et al. (2016). Oncotarget. 7(38); 62292-62304; Bohnsack, MT., et al. (2004). RNA (2004), 10(2); 185-191).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Exportin-5, clone EX5 4E1, Cat. No. MABE1922, is a rat monoclonal antibody that detects Exportin-5 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Exportin-5 in Western Blotting applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).

      Immunofluorescence Analysis: A representative lot detected Exportin-5 in Immunofluorescence applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).

      ELISA Analysis: A representative lot detected Exportin-5 in ELISA applications (Ott, C.A., et. al. (2016). Oncotarget. 7(38):62292-62304).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Exportin-5.
      EpitopeN-terminal half
      CloneEX5 4E1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone EX5 4E1 is a rat monoclonal antibody that detects human Exportin-5. It targets an epitope within 15 amino acids from the N-terminal half.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • XPO5
      • KIAA1291
      • RANBP21
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~135 kDa observed; 136.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A375 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Exportin-5 in A375 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1922-25UL 04061842414802

      Documentation

      Anti-Exportin-5 Antibody, clone EX5 4E1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Exportin-5 Antibody, clone EX5 4E1 Certificates of Analysis

      TitleLot Number
      Anti-Exportin-5, clone EX5 4E1 - Q3490931 Q3490931

      References

      Reference overviewPub Med ID
      Induction of exportin-5 expression during melanoma development supports the cellular behavior of human malignant melanoma cells
      Corinna Anna Ott 1 , Lisa Linck 1 , Elisabeth Kremmer 2 , Gunter Meister 3 , Anja Katrin Bosserhoff
      Oncotarget  7(38)  62292-62304  2016

      Show Abstract       		27556702 27556702