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05-104 Anti-EGFR Antibody, clone LA22

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05-104
250 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-104
      Replaces04-337; 04-338
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EGFR Antibody, clone LA22
      Alternate Names
      • Receptor tyrosine-protein kinase ErbB-1
      • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
      • cell growth inhibiting protein 40
      • cell proliferation-inducing protein 61
      • epidermal growth factor receptor
      • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
      • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog
      • avian)
      Background InformationProtein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
      Product Information
      FormatPurified
      Control
      • Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
      PresentationPurified mouse monoclonal IgG2a in storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationAnti-EGFR Antibody, clone LA22 is an antibody against EGFR for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated EGF receptor from 500 μg of A431 RIPA lysate.

      Immunocytochemistry:
      5 μg/mL of previous lots of the antibody showed positive immunostaining for EGF receptor in A431 cells fixed with 4% paraformaldehyde followed by 100% methanol.
      Biological Information
      ImmunogenA431 membrane proteins
      CloneLA22
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Product Usage Statements
      Quality AssuranceEvaluatd by RIPA lysates from human A431 cells under reducing conditions.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected EGF receptor in RIPA lysates from human A431 cells under reducing conditions.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size250 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-104 04053252623691