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MABT322 Anti-DDR2 Antibody, clone 2B12.1

This Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.

Anti-DDR2 Antibody, clone 2B12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: DDR2, NTRKR3, TKT, TYRO10 UniProt Number: Q16832
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MABT322
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT322
      DescriptionAnti-DDR2 Antibody, clone 2B12.1
      Alternate Names
      • Discoidin domain-containing receptor 2
      • CD167 antigen-like family member B
      • CD167b
      • Discoidin domain receptor 2
      • Discoidin domain-containing receptor tyrosine kinase 2
      • Neurotrophic tyrosine kinase, receptor-related 3
      • Receptor protein-tyrosine kinase TKT
      • Tyrosine-protein kinase TYRO10
      Background InformationDiscoidin domain-containing receptor 2 (EC 2.7.10.1; UniProt Q16832; also known as CD167 antigen-like family member B, CD167b, Discoidin domain receptor 2, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10) is encoded by the DDR2 (also known as NTRKR3, TKT, TYRO10) gene (Gene ID 4921) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR2 is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature protein with an extracellular region (a.a. 22-399), followed by a transmembrane segment (a.a. 400-421) and a cytoplasmic region (a.a. 422-855) that harbors the kinase domain (a.a. 563-849).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of rat L6 myoblast and human HeLa cell lysates.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected DDR2 in human lung and kidney tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human DDR2 internal fragment.
      EpitopeInternal (C-terminal half).
      Clone2B12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DDR2
      • NTRKR3
      • TKT
      • TYRO10
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~107/115 kDa observed. Target band(s) appears larger than the calculated molecular weight of 96.74 kDa due to glycosylation. A doublet banding pattern is often seen with the lower band representing less glycosylated DDR2 species.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT322 04055977314960