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ABN289 Anti-Cullin-5 (CUL5) Antibody

Detect Cullin using this rabbit polyclonal antibody, Anti-Cullin-5 (CUL5) Antibody validated for use in western blotting & IHC.

Anti-Cullin-5 (CUL5) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: CUL5, VACM1 UniProt Number: Q93034
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ABN289
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN289
      ReplacesAB10622
      DescriptionAnti-Cullin-5 (CUL5) Antibody
      Alternate Names
      • Cullin-5
      • CUL-5
      • Vasopressin-activated calcium-mobilizing receptor 1
      • VACM-1
      Background InformationCUL-5/VACM-1 (cullin-5, vasopressin-activated calcium mobilizing receptor 1) is one component of the ECS (elongin B/C-cul2/cul5-SOCS box protein) complex that forms an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome. CUL-5 is ubiquitously expressed in the central nervous system and in many peripheral organs. Human immunodeficiency virus-1 (HIV-1) protein Vif forms a Skp1-cullin-F-box (SCF)-like complex with CUL-5/VACM-1 and Elongins B and C. The ability of Vif to suppress the antiviral activity of APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is specifically dependent on CUL-5-SCF function, as it allows Vif to interact with APOBEC3G and induce its ubiquitination and degradation. The CUL-5-SCF pathway used by Vif is thus a potential target for antiviral development. CUL-5/VACM-1 inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways. In human tissues expression in kidney, brain, heart, placenta, and skeletal muscle is observed. CUL-5/VACM-1 is also expressed in both breast epithelial cells and breast cancer cells. Decreased expression of CUL-5/VACM-1 may play a role in breast tumorigenesis. Further evidence for this finding is observed in T47D breast cancer cells in which overexpression of CUL-5/VACM-1 significantly attenuates cellular proliferation and MAPK phosphorylation when compared to control cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human heart tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect Cullin using this rabbit polyclonal antibody, Anti-Cullin-5 (CUL5) Antibody validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cullin-5 in human placenta and human testis tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Cullin-5 near the Near N-Terminus.
      EpitopeNear N-Terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Cullin-5 near the N-Terminus.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rabbit based on 100% sequence homology. Other homologies: Feline (93% sequence homology) and Mouse and Rat (87% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CUL5
      • VACM1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~90 kDa observed. Neddylation of Cullin-5 at a.a. 724 with NEDD8 results in a 10 kDa increase in molecular weight (Hori. T., et al. (1999). Oncogene. 18(48):6829-6834).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-5 in 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN289 04053252914690