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ABT45-25UG Anti-Claudin-5 (rabbit polyclonal)

Anti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P).

Anti-Claudin-5 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CLDN5, AWAL, TMVCF UniProt Number: O00501
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ABT45-25UG
25 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Eq, OxWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT45-25UG
      DescriptionAnti-Claudin-5 (rabbit polyclonal)
      Alternate Names
      • Claudin-5
      • Transmembrane protein deleted in VCFS
      • TMDVCF
      Background InformationClaudin-5, a transmembrane protein deleted in velo-cardio-facial syndrome (TMVCF), seems to be involved in endothelial tight junctions. Claudin-5 has been exclusively found in the cell-cell borders of endothelial cells. Lower claudin-5 levels in sinusoidal endothelial cells could lead to their dysfunction during liver injuries. Levels of claudin-5 in tumor vessels could be a potential marker for hepatocellular carcinoma prognosis. The loss of claudin-5 function has been linked to carcinogenesis.
      Product Information
      FormatAffinity Purified
      Control
      • Human lung tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:800 dilution from a representative lot detected Clausin-5 in prostate tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Claudin-5 at the cytoplasmic domain.
      EpitopeCytoplasmic Domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes Claudin-5 at the cytoplasmic domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Ox
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat.
      Predicted to react with Equine and Ox based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • CLDN5
      • AWAL
      • TMVCF
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a major role in tight junction-specific obliteration of the intercellular space (By similarity).

      SUBUNIT STRUCTURE: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).

      SUBCELLULAR LOCATION: Cell junction › tight junction. Cell membrane; Multi-pass membrane protein.

      SEQUENCE SIMILARITIES: Belongs to the claudin family.
      Molecular Weight~17 kDa observed.
      Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human lung tissue lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABT45-25UG 04054839350337