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04-932 Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal

This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: CALM, CALML2 , CAM , CAM1, CAM2 , CAM3 , CAMB, CAMC, CAMI, CAMIII, CaM, DD132, PHKD UniProt Number: P62158
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04-932
100 µl  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-932
      Replaces05-932
      DescriptionAnti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal
      Alternate Names
      • calmodulin 1
      • calmodulin 1 (phosphorylase kinase, delta)
      • phosphorylase kinase, delta subunit
      Background InformationCalmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Samples containing the calmodulin binding protein epitope tag.
      PresentationCultured supernatant with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.
      EpitopeCBP epitope
      CloneC16T
      HostRabbit
      SpecificityRecombinant proteins containing the calmodulin binding protein epitope tag.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene SummaryCalmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4
      calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]
      Gene Symbol
      • CALM
      • CALML2
      • CAM
      • CAM1
      • CAM2
      • CAM3
      • CAMB
      • CAMC
      • CAMI
      • CAMIII
      • CaM
      • DD132
      • PHKD
      UniProt Number
      UniProt SummaryFUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
      SUBUNIT STRUCTURE: Interacts with MYO1C and RRAD By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.
      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
      PTM: Ubiquitination results in a strongly decreased activity By similarity.Phosphorylation results in a decreased activity By similarity.
      MISCELLANEOUS: This protein has four functional calcium-binding sites.
      SEQUENCE SIMILARITIES: Belongs to the calmodulin family.
      Contains 4 EF-hand domains.
      Molecular WeightThe molecular weight is related to the tagged protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on lysates from transformed E. coli.
      Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-932 04053252469435

      Documentation

      Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2026403 2026403
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2122450 2122450
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2192168 2192168
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 2239616 2239616
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - 3994709 3994709
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T - NRG1674325 NRG1674325
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T Monoclonal Antibody 2971858
      Anti-Calmodulin Binding Protein Epitope Tag, clone C16T Monoclonal Antibody 2872332