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MABC691 Anti-CRTC1 Antibody, clone 1B5

Detect CREB-regulated transcription coactivator 2 using this mouse monoclonal antibody, Anti-CRTC1 Antibody, clone 1B5 validated for use in western blotting, ICC & Flow Cytometry.

Anti-CRTC1 Antibody, clone 1B5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MECT1, TORC1, WAMTP1, FLJ14027, KIAA0616, CRTC1 UniProt Number: Q6UUV9
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MABC691
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, FCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC691
      DescriptionAnti-CRTC1 Antibody, clone 1B5
      Alternate Names
      • CREB-regulated transcription coactivator 1
      • Mucoepidermoid carcinoma translocated protein 1
      • Transducer of regulated
      • cAMP response element-binding protein 1
      • TORC-1
      • Transducer of CREB protein 1
      Background InformationCREB-regulated transcription coactivator 1 (CRTC1) or in older literature, Mucoepidermoid carcinoma translocated protein 1 or Transducer of regulated cAMP response element-binding element 1 or Transducer of regulated CREB activity 1 (TORC1), is encoded by the human gene named CRTC1/KIAA0616/MECT1, TORC1, or WAMTP1, and CRTC1 is a transcriptional coactivator for CREB1 protein which activates transcription through binding cAMP response element (CRE) sites on DNA. CRTC1 appears to interact with many signaling and metabolic pathways through its regulation of CREB and thus, CRTC1 is involved in diverse physiological processes including energy homeostasis, cancer and endoplasmic reticulum stress. CRTC1 is a powerful activator of PGC1alpha expression and critical for mitochondrial biogenesis in muscle cells. CRTC1 function also varies by cell type. For example, in muscle cells CRTC1 is critical for mitochondrial development, in hippocampal neurons CRTC1 is important for the maintenance of L-LTP (late phase long-term potentiation), and in cortical neurons CRTC1 is important for dendritic spine growth and early neuronal development. CRTC1 is nuclear localized when dephosphorylated and active. CRTC1 is widely expressed in tissues but highest in developing fetal and adult brain, specifically prefrontal cortex and cerebellum. Interestingly recent research on CRTC1 in worms suggests that inhibiting CRTC1 either through phosphorylation or translation extends lifespan significantly, and thus CRTC1 may be important in combating aging and its effects as well. EMD-Millipore Anti-CRTC1 mouse monoclonal antibody has been tested in western blot against recombinant CRTC1 protein and in fluorescent immunocytochemistry on U251 and NTERA2 cell in culture. In addition the antibody has been successfully tested in flow cytometry on K562 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • CRTC1-hIgGFc transfected HEK293 cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CREB-regulated transcription coactivator 2 using this mouse monoclonal antibody, Anti-CRTC1 Antibody, clone 1B5 validated for use in western blotting, ICC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC1 in U251 and NTERA2 cells.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC1 in K562 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human CRTC1 expressed in E. Coli.
      Clone1B5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MECT1
      • TORC1
      • WAMTP1
      • FLJ14027
      • KIAA0616
      • CRTC1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~67 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms between ~63 kDa and ~69 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in CRTC1-hIgGFc transfected HEK293 cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CRTC1 in CRTC1-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC691 04053252916410

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies