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MABN2648-25UG Anti-CHD-8L Antibody, clone 1C10-1

Anti-CHD-8L, clone 1C10-1 , Cat. No. MABN2648, is a rat monoclonal antibody that detects CHD-8L and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

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MABN2648-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2648-25UG
      DescriptionAnti-CHD-8L Antibody, clone 1C10-1
      Alternate Names
      • Chromodomain-helicase-DNA-binding protein 8
      • EC:3.6.4.12
      • ATP-dependent helicase CHD8
      • Axis duplication inhibitor
      • Duplin
      Background InformationChromodomain-helicase-DNA-binding protein 8 (UniProt: Q09XV5; also known as EC:3.6.4.12, CHD-8, ATP-dependent helicase CHD8, Axis duplication inhibitor, Duplin) is encoded by the Chd8 (also known as Kiaa1564) gene (Gene ID: 67772) in murine species. CHD8 is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. It is expressed predominantly from early- to mid-stage mouse embryogenesis and is detected throughout embryos from 7.5 to 9.5 dpc. It localizes to the promoter regions of several β-catenin responsive genes and acts as a negative regulator of Wnt signaling pathway by regulating β-catenin activity. CHD8 is also reported to suppress p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Homozygous deletion of Chd8 gene in mice is reported to result in early embryonic death. Reduced expression of Chd8 gene has been associated with abnormal activation of RE-1 silencing transcription factor (REST), which suppresses the transcription of many neuronal genes. Mice heterozygous for Chd8 mutations exhibit myelination defects and manifest autism spectrum disorder (ASD) with behavioral characteristics that include increased anxiety, repetitive behavior, and altered social behavior. They also exhibit myelination defects Two isoforms of CHD-8 have been reported that are produced by alternative splicing. They are described as a full-length CHD-8L and a CHD-8S that contains only the NH2-terminal chromodomain. (Ref.: Kawamura, A., et al. (2021). Cell Rep. 35(1);108932; Katayama, Y., et al. (2016). Nature. 537(7622); 675-679; Nishiyama, M., et al. (2009). Nat. Cell Biol. 11(2); 172-182).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CHD-8L, clone 1C10-1 , Cat. No. MABN2648, is a rat monoclonal antibody that detects CHD-8L and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunoprecipitation
      Application NotesTested Applications

      Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected CHD-8L in Chromatin Immunoprecipitation applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679; Kawamura, A., et al. (2021). Cell Rep. 35(1);108932).

      Western Blotting Analysis: A representative lot detected CHD-8L in Western Blotting applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679).

      Immunoprecipitation Analysis: A representative lot detected CHD-8L in Immunoprecipitation applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 334 amino acids from the N-terminal region of mouse CHD-8L.
      EpitopeN-terminal
      Clone1C10-1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 1C10-1 is a rat monoclonal antibody that detects Chromodomain-helicase-DNA-binding protein 8 (CHD-8). It targets and epitope within the N-terminal region and is expected to react with isoforms 1 and 2.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Chd8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~290 kDa observed; 290.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E14 Mouse brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHD8L in E14 Mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2648-25UG 04065268528952