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MABE1823-25UG Anti-AsCpf1 Antibody, clone 3D3-F7

Anti-AsCpf1, clone 3D3-F7, Cat. No. MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting.

Anti-AsCpf1 Antibody, clone 3D3-F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas12a, Cpf1 UniProt Number: U2UMQ6
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MABE1823-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BactWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1823-25UG
      DescriptionAnti-AsCpf1 Antibody, clone 3D3-F7
      Alternate Names
      • EC 3.1.27.2
      • AsCpf1
      • CRISPR-associated endonuclease Cpf1
      Background InformationCRISPR-associated endonuclease Cas12a (UniProt: U2UMQ6; also known as EC: 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1) I encoded by the Cas12a (also known as cpf1) gene in Acidaminococcus species. CRISPR is an adaptive immune system that provides protection against mobile genetic elements, such as viruses, transposable elements, and conjugative plasmids. CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. They are transcribed and processed into CRISPR RNA (crRNA). Cas12a, an endonuclease from the type V-A CRISPR system, has several unique characteristics when compared to Cas9 system that increase the utility of CRISPR-based gene editing. Cas12a mediated cleavage utilizes a single and short CRISPR RNA) without requiring trans-activating crRNA. Cas12a recognize a 5' T-rich protospacer adjacent motif (PAM, TTTN for this specific enzyme), unlike Cas9 enzymes which recognize 3' G-rich PAMs, thus this enzyme increases the possibilities for genome editing. The endonuclease activity of Cas12a results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-AsCpf1, clone 3D3-F7, Cat. No. MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
      Clone3D3-F7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
      IsotypeIgG2aκ
      Species Reactivity
      • Bacteria
      Species Reactivity NoteAcidaminococcus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cas12a
      • Cpf1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~151 kDa observed; , 151.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells expressing HA-tagged AsCpf1.

      Western Blotting Analysis: 1 µg/mL of this antibody detected AsCpf1 in lysate from HEK293 cells expressing HA-tagged AsCpf1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1823-25UG 04054839558078