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ABE438 Anti-Apobec1 Antibody

Anti-Apobec1, Cat. No. ABE438, is a rabbit polyclonal antibody that detects Apobec1 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Apobec1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: APOBEC1 UniProt Number: P41238
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ABE438
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE438
      DescriptionAnti-Apobec1 Antibody
      Alternate Names
      • C->U-editing enzyme APOBEC-1
      • EC:3.5.4.36
      • Apolipoprotein B mRNA-editing enzyme 1
      • HEPR
      • mRNA(cytosine(6666)) deaminase 1
      Background InformationC->U-editing enzyme APOBEC-1 (UniProt: P41238; also known as EC:3.5.4.36, Apolipoprotein B mRNA-editing enzyme 1, HEPR, mRNA(cytosine(6666)) deaminase 1) is encoded by the APOBEC1 gene (Gene ID: 339) in human. Apobec1 is a homodimeric protein that is expressed exclusively in mammalian intestines. It is the catalytic component of the apolipoprotein B mRNA editing enzyme complex and serves as a zinc-dependent cytidine deaminase that converts a specific cytidine to uridine in apolipoprotein B (ApoB) mRNA transcripts. Hence, it is responsible for the post-transcriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Its CMP/dCMP-type deaminase domain is localized in amino acids 10-134. Overexpression of Apobec1 has been linked to hepatocellular carcinoma in mice and rabbits and its deficiency in cancer-prone mice is reported to reduce the number of polyps and tumors in the gastrointestinal tract. (Ref.: Saraconi, G., et al. (2014). Genome Biol. 15; Article number 417; Rosenberg, BR., et al. (2011). Nat. Struct. Biol. 18(2); 230-236).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human spleen tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Apobec1, Cat. No. ABE438, is a rabbit polyclonal antibody that detects Apobec1 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:500-2,000 from a representative lot detected Apobec1 in Rat Small Intestine and Human colon tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human C->U-editing enzyme Apobec1.
      EpitopeInternal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects C->U-editing enzyme Apobec1. It targets an epitope within 14 amino acids from the internal region.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APOBEC1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~30 kDa observed; 28.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat Spleen tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Apobec1 in Rat spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE438 04053252909375