Millipore Sigma Vibrant Logo

ST1088 Anti-Akt, PH Domain Mouse mAb (SKB1)

Anti-Akt, PH Domain, mouse monoclonal, clone SKB1, recognizes the ~60 kDa Akt protein in A431 cells. It is validated for flow cytometry, Western blotting, and immunoprecipitation.

Anti-Akt, PH Domain Mouse mAb (SKB1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PKB, Pleckstrin Homology Domain

ST1088
View Pricing & Availability

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
ST1088-1SET
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 1 set
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Add To Favorites
      Description
      OverviewRecognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
    • Antibody Target Gene Symbol: AKT1
    • Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
    • Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
    • Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
    • Mu Entrez ID: 11651
    • Rat Entrez ID: 24185
      		•
      Catalogue NumberST1088
      Brand Family Calbiochem®
      SynonymsAnti-PKB, Pleckstrin Homology Domain
      Application Data
      Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
      Product Information
      FormLiquid
      Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
      Positive controlA431 cells (included)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.
      Key Applications Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesFlow Cytometry (see comments)
      Immunoblotting (0.5-2 µg/ml, see application references)
      Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
      Application CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
      ImmunogenHuman
      CloneSKB1
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      ST1088-1SET 04055977224412