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MABE317 Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01

Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
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MABE317
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      ADB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE317
      DescriptionAnti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01
      Alternate Names
      • 5hmeC
      • 5' hydroxymethyl cytosine
      • 5'OH methyl cytosine
      • 5'OHmeC
      Background Information5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.
      Key Applications
      • Dot Blot
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesHydroxymethylated DNA Immunoprecipitation (hMeDIP) Analysis: A representative lot was used by an independent laboratory to immunoprecipitate 5-Hydroxymethylcytosine DNA usingh chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)
      Biological Information
      ImmunogenBSA-conjugated recombinant Hydroxymethylcytosine DNA.
      CloneHMC-MA01
      HostMouse
      SpecificityThis antibody detects 5-Hydroxymethylcytosine, but not 5-Methylcytosine or unmethylated cytosine.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteExpected to detect 5-Hydroxymethylcytosine in all species
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-Hydroxymethylcytosine DNA.

      Dot Blot Analysis: A 1:5000 dilution of this antibody detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE317 04053252551857

      Documentation

      Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 Certificates of Analysis

      TitleLot Number
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 2930991
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 2458017 2458017
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 3503874 3503874
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - 3760035 3760035
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 - Q2063315 Q2063315
      Anti-5-Hydroxymethylcytosine (5hmC), clone HMC-MA01 Monoclonal Antibody 3113128

      Brochure

      Title
      CpGenome™ 5-hmC Quantitation Kit

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies