Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
The quantity field is empty. Please enter a quantity of 1 or more to add items to your cart.
Description
Overview
Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.
Catalogue Number
164599
Brand Family
Calbiochem®
Synonyms
APN/CD13, aminopeptidase N
References
References
Bhagwat, S.V., et al. 2003. Blood101, 1818. van Hensbergen, Y., et al. 2002. Clin. Cancer Res.8, 3747. Ishii, K., et al. 2001. Biol. Pharm. Bull.24, 226.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-<i>p</i>NA per min at 37°C, pH 7.2
Bhagwat, S.V., et al. 2003. Blood101, 1818. van Hensbergen, Y., et al. 2002. Clin. Cancer Res.8, 3747. Ishii, K., et al. 2001. Biol. Pharm. Bull.24, 226.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-April-2008 RFH
Synonyms
APN/CD13, aminopeptidase N
Description
Native aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.
Form
Liquid
Formulation
In 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
200 mM Tris-HCl, pH 7.2, 100 µl substrate (L-leu-pNA; final concentration 1 mM) in 200 mM Tris-HCl, pH 7.2, enzyme sample, final volume 200 µl; incubate at 37°C for 10 min.*; cleavage is monitored at 405 nm.
*Note: Incubation time of 10 min. is provided as a guideline. The incubation time depends on the activity of the enzyme and the sensitivity of the instrument and should be determined for individual assays.
EC number
3.4.11.2
Purity
≥90% by SDS-PAGE
Specific activity
≥100 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Bhagwat, S.V., et al. 2003. Blood101, 1818. van Hensbergen, Y., et al. 2002. Clin. Cancer Res.8, 3747. Ishii, K., et al. 2001. Biol. Pharm. Bull.24, 226.