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Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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119140
Sigma-AldrichAdenosine Receptor Agonist, NECA - CAS 35920-39-9 - Calbiochem
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Description
Overview
A cell-permeable adenosine analog that acts a potent non-selective agonist of adenosine receptors (Ki = 14 nM, 20 nM, 2.4 µM and 6.2 nM for A1, A2A, A2B, A3, respectively). Increases intracellular cAMP production (EC50 = 3.1 µM in A2B expressing CHO cells). Shown to increase glucagon release in a dose-dependent manner and inhibit insulin release at low concentrations. Although at higher concentration some insulin release is observed. Also, displays a wide range adenosine-dependent effects, such as blocking platelet aggregation and inhibiting DNA synthesis. When administered at reperfusion, it is shown reduce infarction and block the formation of the mitochondrial permeability transition pore by activating p70S6 kinase.
Adenosine Receptor Agonist, NECA - CAS 35920-39-9 - Calbiochem Certificates of Analysis
Title
Lot Number
119140
References
Reference overview
Forster, K., et al. 2006. Basic Res. Cardiol.101, 319. de Zwart, M., et al. 1998. Nucleosides Nucleotides.17, 969. Phillis, J. et al. 1986, Pharm. Biochem. Behavior.24, 263 Durcan, M. J., et al. 1989. Pharmacol Biochem Behav.32, 487. Bacher, S., et al. 1982. Naunyn Schmiedebergs Arch Pharmacol.329, 67. Cusack, N., et al. 1981. Br J Pharm.72, 443. Londos, C., et al. 1980. Proc Natl Acad Sci.77, 2551.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
A cell-permeable adenosine analog that acts a potent non-selective agonist of adenosine receptors (Ki = 14 nM, 20 nM, 2.4 µM and 6.2 nM for A1, A2A, A2B, A3, respectively). Increases intracellular cAMP production (EC50 = 3.1 µM in A2B expressing CHO cells). Shown to increase glucagon release in a dose-dependent manner and inhibit insulin release at low concentrations. Although at higher concentration some insulin release is observed. Also, displays a wide range adenosine-dependent effects, such as blocking platelet aggregation and inhibiting DNA synthesis. When administered at reperfusion, it is shown reduce infarction and block the formation of the mitochondrial permeability transition pore by activating p70S6 kinase.
Form
White solid
Intert gas (Yes/No)
Packaged under inert gas
CAS number
35920-39-9
Chemical formula
C₁₂H₁₆N₆O₄
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO (50 mM)
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Forster, K., et al. 2006. Basic Res. Cardiol.101, 319. de Zwart, M., et al. 1998. Nucleosides Nucleotides.17, 969. Phillis, J. et al. 1986, Pharm. Biochem. Behavior.24, 263 Durcan, M. J., et al. 1989. Pharmacol Biochem Behav.32, 487. Bacher, S., et al. 1982. Naunyn Schmiedebergs Arch Pharmacol.329, 67. Cusack, N., et al. 1981. Br J Pharm.72, 443. Londos, C., et al. 1980. Proc Natl Acad Sci.77, 2551.