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69048 T7•Tag® Antibody HRP Conjugate

69048
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Preis & Verfügbarkeit

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69048-3
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      Kst.-Ampulle 100 ul
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      Description
      OverviewThe T7•Tag Antibody HRP Conjugate is useful as a single detection reagent and eliminates the need for secondary antibodies or streptavidin conjugates.The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

      The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

      This antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase.

      SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
      Species/IsotypeMouse monoclonal IgG2b
      Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
      Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
      < 1 ng (developed with chemiluminescent substrates)
      FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
      Working dilution1:5,000–1:10,000
      Catalogue Number69048
      Brand Family Novagen®
      Product Information
      Components
      Quality LevelMQ100
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Bestellnummer GTIN
      69048-3 04055977259780