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553504 RAD51, His•Tag®, Pneumocystis carinii, Recombinant, E. coli

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553504
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553504-10KU
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      Description
      OverviewRecombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.
      Catalogue Number553504
      Brand Family Calbiochem®
      References
      ReferencesWest, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435.
      Jasin, M. 2002. Oncogene 21, 8981.
      Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630.
      Daboussi, F., et al. 2002. Cell. Signal. 14, 969.
      Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.
      Product Information
      Unit of DefinitionOne unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Quality LevelMQ100
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Bestellnummer GTIN
      553504-10KU 04055977193657