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14-525M PKC ζ Protein, active, 250 µg

Active, N-terminal His6-tagged recombinant human PKC zeta, residues 2-end, for use in Kinase Assays.

PKC ζ Protein, active, 250 µg: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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14-525M
250 µg  Also available in 10 μg size (cat#14-525) and in bulk (cat# 14-525-K).
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      Übersicht

      Replacement Information
      Description
      Catalogue Number14-525M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKC ζ Protein, active, 250 µg
      OverviewN-terminal His6-tagged recombinant human PKC zeta, residues 2-end
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged recombinant human PKC zeta, residues 2-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • PRKCZ
      • OTTHUMP00000044160
      • nPKC-zeta
      • PKC2
      Protein TargetPKCζ
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05513 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
      SIZE: 592 amino acids; 67660 Da
      SUBUNIT: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB.
      SUBCELLULAR LOCATION: Cytoplasm. Endosome. Note=In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes.
      TISSUE SPECIFICITY: Expressed in brain, and to a lesser extent in lung, kidney and testis.
      DOMAIN: SwissProt: Q05513 The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. & The C1 domain does not bind the diacylglycerol (DAG).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 OPR domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
      Molecular Weight68.9kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of PKC substrate peptide II
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-525) and in bulk (cat# 14-525-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      14-525M 04053252338823

      Verwandte Produkte & Anwendungen

      Alternative Packsize

      Bestellnummer Beschreibung  
      14-525 PKC ζ Protein, active, 10 µg Preis & Verfügbarkeit

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      Kategorien

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases