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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

411550 Sigma-AldrichMouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

SDB
Analysenzertifikat
Literatur
Data Sheet

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Übersicht

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Description
Catalogue Number	411550
Brand Family	Calbiochem®

References
References	Hamilton, R.G. 1993. Clin. Chem. 39, 1988. Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107. Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.

Product Information
Form	Liquid
Formulation	In PBS, 10 mg/ml BSA.
Preservative	None
Quality Level	MQ200

Applications
Application References	Enzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Key Applications	Enzyme Immunoassay
Application Notes	Enzyme Immunoassay (1:1000, see application references)
Application Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: 100% Human IgG₂: 100% Human IgG₃: 100% Human IgG₄: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: 100% Human IgG₂: 100% Human IgG₃: 100% Human IgG₄: 100% Human IgM: <0.01% Human IgA: <0.01% Human IgE: <0.01% There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.

Biological Information
Clone	HP6043
Host	Mouse
Isotype	IgG
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Bestellnummer	GTIN
411550-500UG	07790788060275

Documentation

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate SDB

Titel
Sicherheitsdatenblatt (SDB)

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate Analysenzertifikate

Titel	Chargennummer
411550	Chargen-Nr.

Literatur

Übersicht
Hamilton, R.G. 1993. Clin. Chem. 39, 1988. Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107. Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.

Datenblatt

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	11-March-2009 JSW
Application	Enzyme Immunoassay (1:1000, see application references)
Description	Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
Background	The HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the CALBIOCHEM^® technical monograph, The Human IgG Subclasses, is available free of charge.
Host	Mouse
Clone	HP6043
Isotype	IgG
Form	Liquid
Formulation	In PBS, 10 mg/ml BSA.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Antibody should be titrated for optimal results in individual systems. Cross-reactivity by ELISA against human myeloma proteins: Human IgG₁: 100% Human IgG₂: 100% Human IgG₃: 100% Human IgG₄: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% Cross-reactivity by ELISA against chimeric antibodies: Human IgG₁: 100% Human IgG₂: 100% Human IgG₃: 100% Human IgG₄: 100% Human IgM: <0.01% Human IgA: <0.01% Human IgE: <0.01% There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Toxicity	Standard Handling
References	Hamilton, R.G. 1993. Clin. Chem. 39, 1988. Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107. Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
Application references	Enzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.

Kategorien

Life Science Research > Antibodies and Assays > Secondary Antibodies

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