Millicell^® μ-Angiogenesis Activation and Inhibition Assay Kits

Studying how compounds affect angiogenesis, either to promote or inhibit new capillary tube formation can lead to therapies affecting wound healing, tissue regeneration, cardiovascular disease, stroke, tumor progression, and more. The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.

Benefits

• Continuous, live monitoring of cells as tubules form
  
• Even, flat slide surface prevents any liquid meniscus from forming, eliminating out-of-focus areas
  
• Full well visualization at low magnification
  
• Optimized assay conditions in a miniaturized format
  
• Compatible with multi-channel pipettes and fixation reagents
  
• Less reagents and cells needed, reducing costs and waste by up to 80% • All-in-one slide used to grow, assay, and stain cells

Supporting Data

μ-Angiogenesis Inhibition Kit

Increasing concentrations of sulforaphane resulted in both mean tube length and mean number of branch points as shown below in bright field and calcein-AM micrographs of HUVEC cells.

μ-Angiogenesis Activation Kit

(Left to right) Bright field, calcein-AM, DAPI, and calcein-DAPI merge micrographs show robust tubule formation and lumen structure in the presence of phorbol myristate acetate (PMA, a pro-angiogenic agent). Images shown are of HUVEC cells.

Applications

Angiogenesis Assays